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Prolactin ELISA Kit (PRL)

INTENDED USE

Human prolactin ELISA kit is a reliable quantitative procedure for measuring PRL (prolactin, lactogenic hormone) in human serum samples. This assay has a minimum sensitivity detection limit of 0.35 ng/ml.

BACKGROUND

Prolactin (PRL) plays vital roles in the production and secretion of milk, the development of the mammary gland and in controlling the female and male gonadal functions. During pregnancy, the concentration can increase progressively reaching levels that can be between 10-20 times higher than normal values, it can then decline to non-pregnant levels by 3-4 weeks post- partum. However, breast feeding mothers can maintain the high levels and it can take several months for the serum concentrations to return back to similar levels as non-pregnant levels. The primary functions is to initiate breast development and to maintain lactation, it can also suppress gonadal function.

The following assay procedure provides a sensitive, rapid and a reliable assay. It also displays no cross-reactivity with LH, hCG, FSH, hGH and TSH. The plasma concentration of prolactin is known to increases in a number of physiological situations for example during stress, pregnancy and lactation. The physiological levels can also be found to fluctuate according to nycthemeral rhythm, where at night a significant rise can be observed.

Ovulatory suppressants and drugs with anti-dopamine activity (psychotropic agents) can also increase the secretion . The measurement of its concentrations is also useful in aiding the diagnosis of hypothalamic- pituitary disorders. High levels have been linked with galactorrhea and amenorrhea, micro-adenomas (small pituitary tumors) which can cause hyperprolactinemia, which is sometimes linked with male impotence.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells: Break apart strips, coated with anti-prolactin antibody (monoclonal).
  • Standards 0-5: Lyophilized human prolactin. Concentration 0, 5, 20, 50, 100, 200 ng/mL. The standards are calibrated against WHO 3rd International Standard IRP (84/500.).
  • Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase (HRP).
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of prolactin (PRLlactogenic hormone) using current prolactin ELISA kit was approximately 0.35 ng/ml. The dynamic range for this assay is 5.0 – 200.0 ng/ml.

ASSAY CHARACTERISTICS

– Assay Dynamic Range: 0.35 ng/mL ‒ 200.0 ng/mL.
– Specificity (Cross Reactivity): Highly specific for prolactin, no cross reaction with hCG, TSH, LH, FSH was detected.
– Analytical Sensitivity: 0.35 ng/ml
– Intra-Assay Variability: 2.91 – 5.87%
– Inter-Assay Variability: 5.64 – 6.22%
– Recovery: 86.3 – 110.2%
– Linearity: 88.7 – 111.4%
– Drug Interferences: None
– Interfering Substances: None
– High-dose-Hook Effect:  No hook effect detected up to 2000 ng/mL of Prolactin.

REFERENCES

  1. New insights in prolactin: pathological implications. Nat Rev Endocrinol. (2015) 11 (5): 265-75. Review. Bernard V., et al.
  2. Prolactin–not only lactotrophin. A “new” view of the “old” hormone. J Physiol Pharmacol. (2012) 63 (5): 435-43. Review. Ignacak A., et al.
  3. Prolactin and human tumourogenesis. J Neuroendocrinol. (2010) 22 (7): 771-7. Review. Fernandez I., et al.
  4. The role of prolactin in andrology: what is new? Rev Endocr Metab Disord. (2015) 16 (3): 233-48. Review. Rastrelli G., et al.

ADDITIONAL INFORMATION

  • Full Name: Prolactin ELISA Kit (PRL)
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.35 ng/ml

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