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Vitamin H (Biotin) ELISA Kit

  • Created on the 20 October, 2017.

BACKGROUND

Vitamin H, which is also more frequently called biotin, belongs to the family of B complex group of vitamins. These vitamins play essential roles in converting food (usually carbohydrates, amino acids and fats) into fuel (glucose) and this is used in order to produce energy. B vitamins are water soluble (i.e. the body is unable to store them), however, biotin can be produced by bacteria within the intestines and small amounts can be found in many foods. Some of the common foods include: cooked eggs, sardines, brewer’s yeast, nuts (pecans, almonds, walnuts and peanuts), soybeans, whole grains, banana, mushroom, cauliflower and other legumes (such as blackeye peas and beans). Many food-processing techniques have been found to destroy biotin, whereas less-processed versions of the same foods are found to contain more biotin. Also, raw egg whites are known to contain a protein called avidin and this protein is able to interfere with the ability of the body to absorb biotin. It is also recommended for strengthening nails and hair and is often found to be added to many cosmetic products for skin and hair. Biotin plays a vital role for normal embryonic growth and therefore is an important nutrient during pregnancy. When there is a lack of biotin in the body this results in nervous disorders, muscle pain, seborrhoea, dermatitis, tiredness and anorexia. This biotin ELISA is intended for measuring quantitative levels of vitamin H (biotin) in food.

INTENDED USE

Vitamin H ELISA kit is designed for detecting quantitative concentrations of biotin (vitamin H) in food. This assay has a minimum analytical sensitivity limit of 0.5 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of biotin (vitamin H) using this vitamin H ELISA kit was 0.5 ng/ml. The standard assay range for this kit is 1.0 – 25.0 ng/ml.

REFERENCES

  1. Biotin: biochemical, physiological and clinical aspects. Subcell Biochem. (2012) 56: 1-19. Review. Said H.M.
  2. Vitamin H-regulated transgene expression in mammalian cells. Nucleic Acids Res. (2007) 35 (17): e116. Weber W., et al.
  3. A versatile Escherichia coli strain for identification of biotintransporters and for biotin quantification. Bioengineered. (2014) 5 (2): 129-32. Finkenwirth F., et al.
  4. Adequate intake of biotin in pregnancy: why bother? J Nutr. (2014) 144 (12): 1885-6. Mock D.M.
  5. Solid-phase synthesis of a biotin derivative and its application to the development of anti-biotin antibodies. Appl Biochem Biotechnol. (2010) 162 (1): 221-32. Papasarantos I., et al.

ADDITIONAL INFORMATION

  • Full Name: Vitamin H (Biotin) ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.5 ng/ml

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