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Vitamin B12 ELISA Kit

INTENDED USE

Vitamin B12 ELISA kit is designed for detecting quantitative concentration of vitamin B12 (folate) in food. This assay has a minimum analytical sensitivity limit of 0.3 ng/ml.

BACKGROUND

Vitamin B12 or B9 (which is also often referred to as folate) are trace elements that belong to a group of vital chelate formers. It is able to form stable complexes that are able to be absorbed through the lower section of the small intestine (along with the intrinsic factor that are present in the gastric juice). The following vitamin B12 ELISA kit offer a more sensitive and quicker procedure for determining quantitative levels of vitamin B12 present in vitaminated food when compare to other conventional microbiological methods.

Vitamin B12 deficiency can result in anemia and many other neurological dysfunction. Mild deficiencies often do not show any discernible symptoms, however, over time these become more significant and can eventually result in anemia. Symptoms of anemia include: light-headedness, fatigue, rapid breathing, weakness and pale coloured skin. The condition of anemia causes the body to produce fewer red blood cells than normal or abnormally low amount of a haemoglobin which ultimately leads to red blood cells unable to function properly. A pernicious anemia can be easily treated by the administration of high dosage of vitamin B12.

VITAMIN B12 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with anti vitamin B12.
  • Vitamin B12 Standards 1-6: Concentration 0, 0.4, 1, 4, 10, 40 ng/ml.
  • Conjugate (Vitamin B12-Peroxidase).
  • Substrate Solution (TMB).
  • Stop Solution (1 N acidic solution).
  • Sample Diluent (PBS).
  • Washing Solution (PBS + Tween 20)(10x Concentrate).
  • Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of vitamin B12 (folate) using current vitamin B12 ELISA kit was 0.3 ng/ml. The standard range for this assay is 0.4 – 40.0 ng/ml.

ASSAY CHARACTERISTICS

– Vitamin B12: ELISA
– Sensitivity: 0.3ng/ml (based on the standard curve).
– Recovery: 98% based on spiked samples.
– Intra-Assay Precision: 3%
– Cross-Reactivity: Relative to vitamin B12 (=100%): Hydroxycobalamine (29%).

REFERENCES

  1. Comparison of five commercially available ELISA kits for the determination of intrinsic factor antibodies in a vitamin B12 deficient adult population. Int J Lab Hematol. (2016) 38 (1): e12-4. Berth M., et al.
  2. Competitive immunoassay for analysis of vitaminB (12). Anal Biochem. (2011) 418 (2): 238-46. Selva Kumar L.S and Thakur M.S.
  3. Iron and vitamin B12 deficiency anaemia in a vegetarian: a diagnostic approach by enzyme-linked immunosorbent assay and radioimmunoassay. Dent Update. (2002) 29 (5): 223-4. Iamaroon A., et al.
  4. Homogeneous enzyme-linkedassay for vitamin B12. Anal Chem. (1987) 59 (6): 837-41. Tsalta C.D. and Meyerhoff M.E.
  5. Relation between erythropoietin and vitamin B12 in normal and anemic pregnant women. Gynecol Obstet Invest. (1995) 39 (2): 83-7. Carretti N., et al.

ADDITIONAL INFORMATION

  • Full Name: Vitamin B12 ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.3 ng/ml

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