VEGF ELISA Kit (Rat)
INTENDED USE
Rat VEGF ELISA kit can be used for analysing quantitative concentrations of rat vascular endothelial cell growth factor (VEGF) in EDTA-plasma and supernatant of cell culture media. This assay has a minimum sensitivity detection limit of 5.44 pg/ml.
BACKGROUND
Vascular endothelial cell growth factor which is commonly referred to as VEGF for short is a homodimeric protein that was originally discovered in bovine conditioned pituitary folliculo-stellate cells. This protein is identical to vascular permeability factor (VPF) and shares many of the common activities displayed by this factor such as stimulation of endothelial cell growth, angiogenesis and capillary permeability. The expression of VEGF in normal human tissues is present in many cells such as: renal glomerular visceral epithelium, hepatocytes, embryonic fibroblasts, Leydig cells, smooth muscle cells, mesangial cells, bronchial and choroids plexus epithelium, activated macrophages and renal glomerular visceral epithelium.
RAT VEGF ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Pre-Coated Plate: Anti-rat VEGF (V-N) rabbit IgG.
- Labelled Antibody (30x Conc.).
- Standards (0-8): Concentration 0, 31.25, 62.5, 125, 250, 500, 1,000, 2,000 pg/ml
- EIA Buffer.
- Solution For Labelled Antibody.
- Chromogen Solution (TMB).
- Wash Buffer (40x Concentrated).
- Stop Solution.
SENSITIVITY
The minimum sensitivity detection limit of vascular endothelial cell growth factor using current rat VEGF ELISA kit is 5.44 pg/ml. The dynamic range for this assay is 31.25 – 2,000.0 pg/ml.
ASSAY CHARACTERISTICS
– Rat Vascular Endothelial Cell Growth Factor (VEGF): ELISA
– Titer Assay: 87.4 – 96.3%
– Added Recovery Assay: 99.66 – 106.95%
– Intra – Assay: 5.4 – 9.3%
– Inter – Assay: 2.4 – 8.6%
– Specificity (Cross Reaction): Rat VEGF 164 (100.0%), Human VEGF 165 (≦0.1%)
– Sensitivity: 5.44 pg/ml
REFERENCES
- Cancer therapy modulates VEGF signaling and viability in adult rat cardiac microvascular endothelial cells and cardiomyocytes. J Mol Cell Cardiol. (2012) 52 (5): 1164-75. Chiusa M., et al.
- Lactotrophs: the new and major source for VEGF secretion and the influence of ECM on ratpituitary function in vitro. Oncol Rep. (2015) 33 (5): 2129-34. Alfer J., et al.
- Effects of vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF) on haemodynamics and permselectivity of the isolated perfused rat kidney. Nephrol Dial Transplant. (1998) 13 (4): 875-85. Klanke B., et al.
- A highly conserved vascular permeability factor secreted by a variety of human and rodent tumor cell lines. Cancer Res. (1986) 46 (11): 5629-32. Senger D.R., et al.
ADDITIONAL INFORMATION
- Full Name: VEGF ELISA Kit (Rat)
- Reactivity: Rat
- Sample Type: EDTA-Plasma, Supernatant Cell Culture Media
- Sensitivity: 5.44 pg/ml
