Rat VEGF ELISA Kit (Vascular Endothelial Growth Factor)

Full Name: Rat VEGF ELISA Kit (Vascular Endothelial Growth Factor)
Reactivity: Rat
Sample Type: EDTA-Plasma, Supernatant Cell Culture Media
Sensitivity: 5.44 pg/ml

BACKGROUND

VEGF is a member of platelet-derived growth factors, it is crucial in regulating the development of blood vessels. It is made up of a domain with four conserved cysteines and a C-terminal heparin-binding region, which are two separate components. Because of this, it can encourage mitosis. Heparin’s affinity for two different domains stabilises the protein’s structural integrity. The protein molecule can keep its form and function because of this binding. It is a non-metastatic protein and has no enzymatic activity. This allows for VEGF to regulate blood vessel development during embryonic development as well as in the adult body. It is produced by endothelial cells in vascular tissues where oxygenated blood is delivered to the cells.

When a form of VEGF, known as Ang-2 is present in the blood, it increases vascular permeability and facilitates blood flow. The 80th amino acid is lysine which is then modified with two acetyl groups at the carboxyl and amine nitrogens that are attached to sulfate esters – a process known as sulfation. These acetyl groups serve to attach the protein to the high-affinity binding sites on the endothelial cells, which are rich in sulfated glycans. In turn, these high-affinity sites allow VEGF to bind with other molecules in order to fullfill its function. The natural VEGF ligands are Ang-1 and Ang-2.

The way VEGF functions is by attaching to receptor molecules on the surface of endothelial cells, which sets off a series of intracellular processes that finally result in the development of new blood vessels (angiogenesis). and blood flow. VEGF-receptors are found in a number of tissues, including corneal and heart tissue. This suggests that the levels are regulated by various factors.

Accordingly, it is contemplated that the levels of VEGF, and/or its receptor, and/or related cytokines can be at least partially regulated, for example in aged animals by administration of one or more pharmaceutical agents. In addition to decreasing macular degeneration risk associated with aging, other beneficial effects of increasing levels of VEGF and/or its receptor are also contemplated to include improved cognitive function in aged animals. In some embodiments of the invention, administering a cyclic nucleotide inhibitor decreases levels of VEGF by at least 25% as compared with non-treated animals.

INTENDED USE

Rat VEGF ELISA kit can be used for analysing quantitative concentrations of vascular endothelial growth factor (VEGF) in rat EDTA-plasma and supernatant of cell culture media. This assay has a minimum sensitivity detection limit of 5.44 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti rat VEGF (V-N) rabbit IgG.
• Labelled Antibody (30x Conc.).
• Standards (0-8): Concentration 0, 31.25, 62.5, 125, 250, 500, 1,000, 2,000 pg/ml
• EIA Buffer.
• Solution For Labelled Antibody.
• Chromogen Solution (TMB).
• Wash Buffer (40x Concentrated).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of rat vascular endothelial cell growth factor using current rat VEGF ELISA kit is 5.44 pg/ml. The dynamic range for this assay is 31.25 – 2,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 87.4 – 96.3%
– Added Recovery Assay: 99.66 – 106.95%
– Intra – Assay: 5.4 – 9.3%
– Inter – Assay: 2.4 – 8.6%
– Specificity (Cross Reaction): Rat VEGF 164 (100.0%), Human VEGF 165 (≦0.1%)
– Sensitivity: 5.44 pg/ml

REFERENCES

1. Cancer therapy modulates VEGF signaling and viability in adult rat cardiac microvascular endothelial cells and cardiomyocytes. J Mol Cell Cardiol. (2012) 52 (5): 1164-75. Chiusa M., et al.
2. Lactotrophs: the new and major source for VEGF secretion and the influence of ECM on ratpituitary function in vitro. Oncol Rep. (2015) 33 (5): 2129-34. Alfer J., et al.
3. Effects of vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF) on haemodynamics and permselectivity of the isolated perfused rat kidney. Nephrol Dial Transplant. (1998) 13 (4): 875-85. Klanke B., et al.
4. A highly conserved vascular permeability factor secreted by a variety of human and rodent tumor cell lines. Cancer Res. (1986) 46 (11): 5629-32. Senger D.R., et al.

OTHER RELATED ELISA KITS

• VEGF ELISA Kit (Mouse)
• TGF-Alpha ELISA Kit
• VEGF ELISA Kit