Human Varicella zoster IgG ELISA kit is designed for analysing in-vitro quantitative concentrations of human IgG class antibodies against Varicella zoster virus (Varicella-IgG) using serum or plasma. This assay has a minimum analytical sensitivity limit of 1.01 U/ml.
Varicella-zoster virus (VZV) is an infectious disease which can cause chickenpox (varicella) during childhood and herpes zoster (singles) during adulthood. VZV is also referred to by many names, such as varicella virus, zoster virus, chickenpox virus and human herpesvirus type 3 (HHV-3). VZV predominately multiplies within the lungs and it can cause a number of different symptoms (pneumonia, bronchitis and encephalitis). Following the primary infection of chickenpox, the virus is found to go dormant in the nerve cells (examples of these include dorsal root ganglia, cranial nerve ganglia and autonomic ganglia). VZV is also very closely related to the herpes simplex viruses (HSV), displaying a high level of genome homology. The virons have a diameter of 180–200 nm, a lipid envelope enclosing the nucleocapsid and the DNA is a double stranded molecule which is 125,000 nucleotides long. Serological screen can be performed to test for IgG-class antibodies to VZV, this can help to identify non-immune individuals. Also, the identification of IgM-class antibodies to VZV can indicate a recent or acute infection but this needs to be correlated with clinical presentation.
VARICELLA ZOSTER IGG ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Varicella zoster antigen coated microtiter strips (IgG).
- Calibrators (A-D): Negative, Cut-Off, Weak Positive and Positive Controls.
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Sample Diluent.
- Washing Buffer (10x).
The minimum detection sensitivity level of IgG antibodies to Varicella zoster virus (Varicella-IgG ) using current Varicella IgG ELISA kit was 1.01 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.
– Varicella Zoster ELISA: IgG
– Intra-Assay-Precision: 7.9 %
– Inter-Assay-Precision: 8.8 %
– Inter-Lot-Precision: 4.2 – 9.1 %
– Analytical Sensitivity: 1.01 U/mL
– Recovery: 81 – 107 %
– Linearity: 70 – 132 %
– Cross-Reactivity: No cross-reactivity to Mumps and Measles.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 94 %
- Reduced varicella-zoster-virus (VZV)-specific lymphocytes and IgG antibody avidity in solid organ transplant recipients. Vaccine. (2013) 31 (20): 2420-6. Prelog M., et al.
- Serological detection of specific IgG to varicella-zoster virus by novel ELISA based on viral glycoprotein antigen. Clin Lab. (2009) 55 (1-2): 1-7. Sauerbrei A. and Wutzler P.
- Varicella-zoster virus-specific, cell-mediated immunity with interferon-gamma release assay after vaccination of college students with no or intermediate IgG antibody response. J Med Virol. 2015 Feb;87(2):350-6. Terada K., et al.
- Serologic analysis of the IgG antibody response in children with varicella zoster virus wild-type infection and vaccination. Pediatr Infect Dis J. (2012) 31 (11): 1148-52. Jenke A.C., et al.
- Comparison of fifteen commercial assays for detecting Varicella Zoster virus IgG with reference to a time resolved fluorescence immunoassay (TRFIA) and the performance of two commercial assays for screening sera from immuno-compromised individuals. J Virol Methods. (2009) 155 (2): 143-9. Chris Maple P.A., et al.
- Full Name: Varicella Zoster IgG ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Serum
- Sensitivity: 1.01 U/ml