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Tumor Protein 53 (P53) ELISA Kit

  • Created on the 28 March, 2017.

BACKGROUND

P53 which can also be called tumor protein 53, cellular tumor antigen p53, protein 53, phosphoprotein p53, transforming related protein p53 and tumor suppressor p53, in humans has several domains, is 393 amino acids long and runs as a 53 kDa protein on SDS-PAGE. It is essentially a tumor suppressor protein which in human is encoded by the TP53 gene and can be found mapped to chromosome 17. It can be activated in response to myriad stressors, such as oxidative stress, DNA damaging agents (UV, IR and many other chemical agents), osmotic shock, deregulated oncogene expression and ribonucleotide depletion. One of the most critical event leading to the activation of p53 is the phosphorylation of its N-terminal domain. This N-terminal transcriptional activation domain has a large number of phosphorylation sites and these are viewed as the primary target for protein kinases transducing stress signals.

The TP53 gene is frequently identified as a mutated gene (greater than 50%) in human cancers and this has led to associating TP53 gene to playing a crucial role in preventing cancer formation. This gene also encodes proteins that have the ability to bind to DNA and help to regulate gene expression in order to prevent mutations of the genome. The p53 molecule plays vital roles in apoptosis, inhibiting angiogenesis, genomic stability and a number of mechanisms that are involved in anti-cancer functions.

INTENDED USE

Human P53 ELISA kit is intended for analysing in vitro quantitative concentrations of tumor protein 53 (P53) in human cell lysates. This assay has a minimum detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of tumor protein 53 (P53) using this human P53 ELISA kit was 10.0 pg/ml. The dynamic assay range for this kit is 156.0 – 10,000 pg/ml.

REFERENCES

  1. p53: an overview of over two decades of study. Malays J Pathol. (2001) 23 (1): 9-16. Review. Cheah P.L. and Looi L.M.
  2. The stress protein TP53INP1 plays a tumor suppressive role by regulating metabolic homeostasis. Biochimie. (2015) 118: 44-50. Review. Saadi H., et al.
  3. Transcriptional regulation by mutant p53 and oncogenesis. Subcell Biochem. (2014) 85: 91-103. Review. Santoro R., et al.
  4. A quantitative overview of radiosensitivity of human tumor cells across histological type and TP53 status. Int J Radiat Biol. (2008) 84 (4): 253-64. Review. Williams J.R., et al.
  5. Clinical implications of p53 mutations in lung cancer. Methods Mol Med. (2003) 75: 53-77. Review. Campling B.G. and el-Deiry W.S.
  6. p53 and apoptosis. Br Med Bull. (1997) 53 (3): 522-38. Review. Bellamy C.O.

ADDITIONAL INFORMATION

  • Full Name: Tumor Protein 53 (P53) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Lysates
  • Sensitivity: < 10.0 pg/ml

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