GET A QUOTE

Tumor Necrosis Factor Alpha (TNF-Alpha) ELISA Kit

  • Created on the 13 February, 2017.

BACKGROUND

Tumor necrosis factor-alpha (TNF-alpha or TNF-α) which is also sometimes called cachectin is a multi-functional cytokine that is secreted by macrophages in response to infection, cancer and inflammation. It has many functions and is involved in a number of different pathways that are important in regulating pathophysiology and homeostasis in mammals. TNF-alpha is primarily expressed in macrophages, however, there are lots for evidence suggesting that it can also be expressed in a number of other cells including: endothelial cells, monocytes, mast-cells, NK-cells, activated lymphocytes, neutrophils and fibroblasts cells.

The human TNF cDNA is 1585 base pairs in length and is encoding a 233 amino acids protein. It was first purified from HL-60 leukemia cells as a 17.3-kilodalton protein, where it displayed cytostatic and cytotoxic activities against a number of different human tumor cell lines. TNF-alpha is located human chromosome 6 and the gene is a member 2 of the TNF-superfamily (there are currently more the 20 distinct members so far).

INTENDED USE

Human TNF-alpha ELISA kit is a reliable quantitative procedure for measuring tumor necrosis factor-alpha (TNF-alpha or TNF-α) in human serum, plasma (EDTA, heparin, citrate) and cell culture medium. This assay has a minimum sensitivity detection limit of 5.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of tumor necrosis factor-alpha (TNF-α) using this human TNF-alpha ELISA kit was approximately 5.0 pg/ml. The dynamic assay range for this kit is 23 – 1,500 pg/ml.

REFERENCES

  1. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor. Proc. Nat. Acad. Sci. (1986) 83: 7467-7471. Broudy, V.C., et al.
  2. Tumor necrosis factor-α signaling in macrophages. Crit Rev Eukaryot Gene Expr. (2010) 20 (2): 87-103. Review. Parameswaran N. and Patial S.
  3. Molecular cloning of the complementary DNA for human tumor necrosis factor. Science (1985) 228: 149-154. Wang, A.M., et al.
  4. TNF-alpha and obesity. Curr Dir Autoimmun. (2010) 11: 145-56. Review. Tzanavari T., et al.

ADDITIONAL INFORMATION

  • Full Name: Tumor Necrosis Factor-Alpha (TNF-Alpha) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA, Citrate, Heparin), Cell Culture Medium
  • Sensitivity: 5.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford