Troponin T (Tn-T) ELISA Kit

  • Created on the 16 May, 2017.


Human troponin T ELISA kit can be used for measuring in vitro quantitative levels of troponin T (TnT) in human cell culture supernatant, plasma, serum, other biological fluids and cell lysates. This assay has a minimum sensitivity limit of 2.54 ng/L.


Troponin T (TnT) is a group of homologous proteins which have a molecular mass between 31-36 kDa and are useful in the laboratory to diagnose heart attack since they are released into the bloodstream when the heart muscles become damaged. Troponin is a myofibrillar protein that is found in striated musculature, it is a 3 protein complex that is made up of troponin C, troponin T and troponin I. It is troponin T which binds the troponin complex to tropomyosin, troponin I which can inhibits actomyosin ATPase and troponin C which mediates calcium dependency through its 4 binding sites for calcium. Troponin T functions in binding to tropomyosin which thereby helps to position it on actin and the rest of the troponin complex is responsible for modulating the contraction of striated muscle. In patients with stable coronary artery disease, the concentrations of troponin T have been significantly associated with incidence of heart failure and cardiovascular death. Elevated levels have also been identified in patients having unstable angina.

In the cytosol, TnT exists either in a protein-bound or free unbound form. The protein bound troponin T is usually released during the later stages of irreversible myocardial damage, whereas, the free (unbound) form is released in the early stages of myocardial damage. Some of the uses for this protein include monitoring acute coronary syndromes and prognosis estimation, the exclusion diagnosis of acute myocardial infarction and the possible use in monitoring patients with nonischemic causes of cardiac injury. Troponin T ELISA kit is designed to be used for various diagnostic applications.


The minimum detection sensitivity level of TnT using current human troponin T ELISA kit was 0.27 ng/L. The dynamic range for this assay is 5.0 – 1,000.0 ng/L.


  1. Troponin T: genetics, properties and function. J Muscle Res Cell Motil. (1998) 19 (6): 575-602. Review. Perry S.V.
  2. The use of cardiac markers in acute coronary syndromes. Scand J Clin Lab Invest Suppl. (1999) 230:50-9. Review. Agrawal B.
  3. The troponin complex and regulation of muscle contraction. FASEB J. (1995) 9 (9): 755-67. Review. Farah C.S. and Reinach F.C.
  4. Role of troponin T in disease. Mol Cell Biochem. (2004) 263 (1-2): 115-29. Review. Gomes A.V., et al.
  5. Structural interactions responsible for the assembly of the troponin complex on the muscle thin filament. Cell Struct Funct. (1997) 22 (1): 219-23. Review. Reinach F.C., et al.


  • Full Name: Troponin T (TnT) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Lysates, Plasma, Other Biological Fluids, Cell Culture Supernatant, Serum
  • Sensitivity: 2.54 ng/L



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford