Triiodothyronine ELISA Kit (T3)
INTENDED USE
Human triiodothyronine ELISA kit can be used to reliably detect levels of triiodothyronine (T3) present in serum or plasma samples. The detection sensitivity of assay is 0.1 ng/ml.
BACKGROUND
Triiodothyronine (T3) have important roles in; regulating metabolism, energy levels, cognitive functions and overall body health. These are essential for normal growth and development and in maintaining a healthy balance of bodily functions.
T3 is transported through the peripheral blood stream as part of its total concentration bound to serum proteins such as thyroxine binding globulin, thyroid binding prealbumin and albumin. It is estimated that only 0.3% of the total T3 circulating in the body is unbound (free fraction). This free fraction is considered to be clinically relevant since it’s believed to have a direct metabolic effect on our bodies.
T3 is a key parameter that is commonly used during the clinical diagnosis and differentiation of thyroid diseases, particularly hyperthyroidism. Additionally, its levels are also successfully used to monitor the effectiveness of treatments for hyperthyroidism.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiterwells, 12 x 8 (break apart) strips: Wells coated with anti T3 antibody.
- Assay Reagent.
- Control Low and Control High.
- Standard 0-5: Concentrations: 0-10.0 ng/ml.
- Substrate Solution: Tetramethylbenzidine (TMB).
- Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase (HRP).
- Stop Solution: 0.5 M H2SO4.
- Wash Solution 40x (Concentrated).
SENSITIVITY
The minimum detectable sensitivity limit of triiodothyronine (T3) using current triiodothyronine ELISA kit was < 0.1 ng/ml. The standard range was calculated to be between 0.5 – 10.0 ng/ml.
REFERENCES
- Clinical usefulness of ELISA technique in the assessment of thyroid function. West Afr J Med (1990) 9(4):258-63. Agharanya JC.
- Thyroid function in very low birth weight infants: effects on neonatal hypothyroidism screening. J Pediatr (1996)128(4):548-54. Frank JE, Faix JE, Hermos RJ, Mullaney DM, Rojan DA, Mitchell ML, Klein RZ.
- Thyroid functions in patients with various chronic liver diseases. Endocrinol Jpn (1988)35(3):357-69. Shimada T, Higashi K, Umeda T, Sato T.
- Total serum levels of triiodothyronine (T3) thyroxine (T4) and thyrotropine (TSH) in school going children of Dibrugarh district: an endemic goiter region of Assam. Indian J Physiol Pharmacol (1997) 41(2):167-70. Thakur C, Saikia TC, Yadav RN.
ADDITIONAL INFORMATION
- Description: Triiodothyronine ELISA Kit (T3)
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 0.1 ng/ml
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