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Trichinella Spiralis IgG ELISA Kit

  • Created on the 25 September, 2017.

BACKGROUND

Trichinella spiralis (also referred to as pork worm) is a nematode parasite which can be found in pigs, horses, rodents, bears and in humans and they are responsible for causing trichinosis disease. Four other species have also been recognised (T. britovi, T. pseudospiralis, T. native and T. nelsoni) that are found worldwide in a large number of omnivorous and carnivorous animals. Trichinella species is the smallest parasite that can be found in humans and it is known to display an unusual lifecycle. The small adult worms are able to mature in the small intestine of the host, each female adult produced many batches of larvae that are able to bore through the intestinal wall before entering the blood stream and the lympahatic system in order to be transported to the striated muscles. Once they have reached the muscle, they become enclosed in a capsule (known as encyst). Humans can be infected by this parasite through eating infected pork, horsemeat and other carnivores (such as cat, fox and bear). The Infection may be detected using methods such as Microscopy, muscle biopsy and Serology (detect antibodies using ELISA assays).

INTENDED USE

Human trichinella spiralis-IgG ELISA kit is designed for measuring in-vitro quantitative concentrations of IgG antibodies against trichinella spiralis using samples of plasma and serum. This assay has a minimum analytical sensitivity limit of > 95.0%.

SENSITIVITY

The minimum detection sensitivity level of trichinella spiralis IgG using this human trichinella spiralis-IgG ELISA kit was greater than 95.0%. The dynamic assay range for this kit is to a specific cut-off point.

REFERENCES

  1. Evaluation of a Western Blot and ELISA for the detection of anti-Trichinella-IgG in pig sera. Vet Parasitol. (2009) 163 (4): 341-7. Nöckler K., et al.
  2. The sequential appearance of IgG subclasses and IgE during the course of Trichinella spiralis infection. Clin Exp Immunol. (1988) 74 (2): 230-5. Ljungström I., et al.
  3. Partial characterization of serine proteinases secreted by adult Trichinella spiralis. Parasitol Res. (2000) 86 (8): 684-7. Todorova V.K. and Stoyanov D.I.
  4. Evaluation of ELISA and Western Blot Analysis using three antigens to detect anti-Trichinella IgG in horses. Vet Parasitol. (2002) 108 (2): 163-78. Pozio E., et al.
  5. Comparison of human trichinellosis caused by Trichinella spiralisand by Trichinella britovi. Am J Trop Med Hyg. (1993) 48 (4): 568-75. Pozio E., et al.

ADDITIONAL INFORMATION

  • Full Name: Trichinella SpiralisIgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: > 95.0%

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