Total Thyroxine (T4) ELISA Kit

  • Created on the 2 February, 2017.


Total thyroxine ELISA kit is a reliable quantitative method designed to detect total thyroxine (T4) concentrations in human serum. This total thyroxine ELISA kit has a detection limit of 8 nmol/l.


Thyroxine (T4) hormone is synthesised and then stored in the thyroid gland, it also represents the major form of the thyroid hormones that are present in blood. These thyroid hormones are important in regulating carbohydrates, protein and fat metabolism and are essential in the development and differentiation of all cells that are found in the human body. Low levels of T4 have been linked with congenital hypothyroidism, chronic thryoiditis (Hashimoto’s disease), myxedema and some genetic abnormalities. Whereas increased levels are found in hyperthyroidism patients that can be due to acute and subacute thyroiditis, Plummer’s disease and Grave’s disease. Higher than 99% of T4 is found to be reversibly bound to one of three plasma binding proteins in blood – thyroxine binding globulin (TBG), albumin and thyroxine binding pre-albumin (TBPA). A small faction, approximately 0.03% of T4 is in the free in its unbound state in blood at any one time and this represent the biological active form. Total thyroxine ELISA kit is intended for many diagnostic applications.


The minimum detectable sensitivity limit of thyroxine (T4) using this total thyroxine ELISA kit was estimated to be 8 nmol/l. Also the standard range for this ELISA assay was calculated to be between 25 – 250 nmol/l.


  1. J. Biological Chemistry (1948) 173, 175. Barker, S.B.
  2. J. Clinical Endocrinol. (1971) 33, 865. Chopra, I.J., Solomon, D.H. and Ho, R.S.
  3. Clinical Chemistry (1975) 21, 3660. Young, D.S., Pestaner, L.C. and Gilberman, U.
  4. Diagnosis and Treatment of Thyroid Disease, Cleveland CRC Press (1975) p. 19-51. Sterling, L.


  • Full Name: Total Thyroxine (T4) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 8 nmol/l



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