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Total Thyroxine ELISA Kit

INTENDED USE

Human total thyroxine ELISA kit is a reliable quantitative method designed to detect total T4 (thyroxine) concentrations in human serum. The sensitivity detection limit of this assay is 8 nmol/l.

BACKGROUND

Thyroxine (T4) hormone is synthesised and then stored in the thyroid gland, it also represents the major form of the thyroid hormones that are present in blood. These thyroid hormones are important in regulating carbohydrates, protein and fat metabolism and are essential in the development and differentiation of all cells that are found in the human body. The following total T4 ELISA kit is intended for many diagnostic applications.

Low levels of thyroxine (T4) have been linked with congenital hypothyroidism, chronic thryoiditis (Hashimoto’s disease), myxedema and some genetic abnormalities. Whereas increased levels are found in hyperthyroidism patients that can be due to acute and subacute thyroiditis, Plummer’s disease and Grave’s disease. This total T4 ELISA kit provides a simple and easy to use tool for the detection of total thyroxine concentrations in various disease conditions. Higher than 99% of thyroxine is found to be reversibly bound to one of three plasma binding proteins in blood – thyroxine binding globulin (TBG), albumin and thyroxine binding pre-albumin (TBPA). A small faction, approximately 0.03% of total T4 is in the free in its unbound state in blood at any one time and this represent the biological active form.

TOTAL THYROXINE ELISA KIT CONETNT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells, 12 x 8 (break apart) strips: Wells coated with human thyroxine (T4) antigen.
  • Enzyme Conjugate: Consisting of anti human thyroxine antibody conjugated to horseradish peroxidase (HRP).
  • Control Low and Control High. Containing human thyroxine (T4).
  • Standard 0-5: Concentration 0-250 nmol/l. Made up from anti human thyroxine.
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution: 0.5 M H2SO4, Avoid contact with the stop solution. It may cause skin irritations and burns.
  • Wash Solution (40x Concentrated).

SENSITIVITY

The minimum detectable sensitivity limit of human total thyroxine (T4) using current total T4 ELISA kit was estimated to be 8 nmol/l. Also the standard range for this ELISA assay was calculated to be between 25 – 250 nmol/l.

REFERENCES

  1. J. Biological Chemistry (1948) 173, 175. Barker, S.B.
  2. J. Clinical Endocrinol. (1971) 33, 865. Chopra, I.J., Solomon, D.H. and Ho, R.S.
  3. Clinical Chemistry (1975) 21, 3660. Young, D.S., Pestaner, L.C. and Gilberman, U.
  4. Diagnosis and Treatment of Thyroid Disease, Cleveland CRC Press (1975) p. 19-51. Sterling, L.

ADDITIONAL INFORMATION

  • Full Name: Total Thyroxine ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 8 nmol/l

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