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Thyroxine (T4) ELISA Kit

  • Created on the 2 February, 2017.

BACKGROUND

Thyroxine (T4) is a hormone that is stored and synthesised in the thyroid gland. The proteolytic cleavage of follicular thyroglobulin results in the releasing T4 into the bloodstream,  over 99% of thyroxine is found to be reversibly bound to at least three different plasma proteins in blood – thyroxine binding globulin (TBG), albumin or thyroxine binding pre-albumin (TBPA). Only a small fraction which represent the active form is found unbound (free) state at any one time. The main function is to act on the body in order to increase the basal metabolic rate by increasing sensitivity to catecholamines (e.g. adrenaline) and by affecting protein synthesis. Hyperthyroidism and thyrotoxicosis are both clinical syndromes which are due to excess levels of circulating free T4, free T3 or in some cases both. Also, both triiodothyronine and thyroxine are known to be useful in treating hypothyroidism (thyroid hormone deficiency).

INTENDED USE

Throxine ELISA kit is a reliable quantitative method to a measuring throxine (T4) in human serum or plasma. This assay has a detection limit of 8.0 nmol/l.

SENSITIVITY

The minimum detectable sensitivity limit of thyroxine (T4) using this throxine ELISA kit was approximately 8.0 nmol/l. The assay dynamic range for this determined to be between 25.0 – 250.0 nmol/l.

REFERENCES

  1. Determination of Protein Bound Iodine. Joumal Biological Chemistry (1948) 173, 175. Barker, S.B.
  2. A Radioimmunoassay of Thyroxine. J. Clinical (1971) EndocrinoL, 33, 865. Chopra, I.J., Solomon, D.H., and Ho, R.S.
  3. Effects of Drugs on Clinical Laboratory Tests. Clinical Chemistry, (1975) 21, 3660. Young, D.S., Pestaner, L.C., and Gilberman, U.
  4. Diagnosis and Treatment of Thyroid Disease, Cleveland CRC Press (1975) P. 19 51. Sterling, L.

ADDITIONAL INFORMATION

  • Full Name: Thyroxine (T4) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity:  8.0 nmol/l

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