Thyroid Peroxidase Antibody (TPO-Ab) ELISA Kit

  • Created on the 2 February, 2017.


Thyroid peroxidase-Ab ELISA kit is a reliable quantitative procedure for measuring thyroid peroxidase antibody (TPO-Ab) in human serum and plasma. This assay has a detection sensitivity limit of 5 IU/ml.


Thyroid peroxidase (TPO) is a membrane-bound hemoprotein which has a molecular weight of nearly 100 kDa. It is essential for thyroid hormone synthesis because this microsomal antigen is an integral membrane protein of the microsomes. Persisting inhibition of TPO activity by specific autoantibodies (Anti-TPO Abs) has been found to cause a decrease in the synthesis of thyroid hormones and thus lead to hypothyroidism. Also, high autoantibodies titers to TPO are frequently discovered in patients that have myxedema (atrophic thyroiditis), Hashimoto’s disease (goitrous thyroiditis) and Graves’ disease. They may be predictive in asymptomatic individuals who are diagnosed for hypothyroidism. Especially at the end of pregnancy, where the determination of thyroid antibodies can be a helpful diagnostic tool to diagnose the early onset of post-partum hypothyroidism.


The minimum sensitivity detection limit of TPO antibody using current thyroid peroxidase-Ab ELISA kit was approximately 5 IU/ml. The dynamic range for this assay is between 33 –  3,000 IU/ml.


  1. Anti-thyroid peroxidase antibody in patients with autoimmune thyroid disease: possible identity with antimicrosomal antibody. J Clin Endocrinol Metab 61 (1985) 1001-1003. Portmann, L., et al.
  2. Thyroid peroxidase is the organ-specific “microsomal” antigen involved in thyroid autoimmunity. Acta Endocrinol (Copenhagen) 281 (1987) 49-55. Ruf, J., et al.
  3. Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J 6 (1987) 4193-4196. Libert, F., et al.
  4. Antithyroid peroxidase autoantibodies in thyroid disease. J Clin Endocrinol Metab 71 (1990) 661-669. Mariotti, S., et al.
  5. Autoimmune thyroid disease. Autoimmunity 37-4 (2004) 337-340. Weetman, A. P.


  • Full Name: Thyroid Peroxidase Antibody (TPO-Ab) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity:  5 IU/ml



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford