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Thrombosis Profile ELISA Kit

  • Created on the 29 June, 2017.

BACKGROUND

There are a complex group of autoantibodies that are linked to thrombosis in connective tissue disease and primary anti-phospholipid syndrome. The antibodies are usually present in patients without lupus but instead with myocardial infarction, arterial and venous thrombosis, recurrent abortion, neurological symptoms and strokes in young people. Even though anti-cardiolipin are most commonly measured, it is also widely accepted that only one group that contains anti-phospholipid autoantibodies with several specificities can be found in these patients. The detection of these antibodies has led to the identification of a new disease entity that has been called anti-phospholipid syndrome (APS). Anti-cardiolipin antibodies which are associated with APS can be used to recognise a serum protein b2-glycoprotein 1 which is also linked with phospholipid. Anti-cardiolipin autoantibodies have the ability to recognise any combination of IgG, IgM and IgA classes. This ELISA assay has the ability to recognise IgG and IgM autoantibodies which are directed against human beta-2-glycoprotein I and phospholipids. However, it must be noted that the antibody concentrations present do not necessary have to correlate with the severity or extent of thrombosis.

INTENDED USE

Human thrombosis ELISA kit is designed for detecting in-vitro quantitative levels of IgG or IgM autoantibodies to phospholipids and beta-2-glycoprotein I in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

Highly purified human beta-2-glycoprotein I, cardiolipin, phosphatidyl inositol, phosphatidyl serine and phosphatidic acid are bound to individual rows of the microwell plate.

SENSITIVITY

The minimum detection sensitivity level of human beta-2-glycoprotein I and phospholipids IgG/IgM autoantibodies using this human thrombosis ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. Management of recurrent thrombosis in antiphospholipid syndrome. Curr Rheumatol Rep. (2014) 16 (3): 405. Review. Nalli C., et al.
  2. Antiphospholipid antibody profile: implications for the evaluation and management of patients. Lupus. (2010) 19 (4): 432-5. Tincani A., et al.
  3. Prevention of thrombosis in antiphospholipid syndrome. Hematology Am Soc Hematol Educ Program. (2016) 2016 (1): 707-713. Review. Lim W.
  4. Primary thrombosis prophylaxis in antiphospholipid antibody-positive patients: where do we stand? Curr Rheumatol Rep. (2011) 13 (1): 59-69. Review. Barbhaiya M. and Erkan D.
  5. Certoparin for the treatment and prevention of thrombosis: pharmacological profile and results from clinical studies. Expert Opin Drug Metab Toxicol. (2013) 9 (7): 901-9. Review. Donadini M.P., et al.

ADDITIONAL INFORMATION

  • Full Name: Thrombosis Profile ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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