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Streptomycin ELISA Kit

  • Created on the 18 October, 2017.

BACKGROUND

Streptomycin is an essential antibiotic which is often used to treat many bacterial infections (for example: tuberculosis, brucellosis, mycobacterium avium complex, tularemia, burkholderia infection, endocarditis and plague). Streptomycin is known to be naturally produced from the actinobacterium Streptomyces griseus and it is also activity is directed against tubercle bacillus and gram-negative bacteria. A number of common side effects include fever, rash, vomiting, heading spinning and numbness of the face. Streptomycin is also used in combination with penicillin as a standard antibiotic cocktail in order to prevent bacterial infection in cell cultures. Due to its many side effects, streptomycin it is rarely used for human treatment, however, it has a vital application in the veterinary area. For example, following the treatment of mastitis in breeding animals, this was found to cause elevated streptomycin in milk, muscle, liver and kidney. Another relatively new application for antibiotic streptomycin is described as Plantomycin (treatment of fruit tree which are known as fire blight).

INTENDED USE

Streptomycin ELISA kit is a procedure designed for determining quantitative amounts of streptomycin in food. This assay has a minimum analytical sensitivity limit of 1.0 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of streptomycin using this streptomycin ELISA kit was 1.0 ng/ml. The standard assay range for this kit is 2.0 – 200.0 ng/ml.

REFERENCES

  1. Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products. Anal Chim Acta. (2011) 701 (2): 209-17. Byzova N.A., et al.
  2. Effect of streptomycin treatment on the shedding of and the serologic responses to Leptospira interrogans serovar hardjo subtype hardjobovis in experimentally infected cows. Vet Microbiol. (1993) 38 (1-2): 129-35. Gerritsen M.J., et al.
  3. Flow-injection determination of streptomycin residues in milk using the luminol-periodate-Mn2+ chemiluminescence system. Luminescence. (2006) 21 (1): 36-42. Wan G.H., et al.
  4. Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J Chromatogr A. (1999) 830 (2): 345-51. Edder P., et al.
  5. One-tube loop-mediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycinin Mycobacterium tuberculosis isolates. J Microbiol Methods. (2010) 83 (1): 53-8. Lee M.F., et al.

ADDITIONAL INFORMATION

  • Full Name: Streptomycin ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.0 ng/ml

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