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SS-B Ab ELISA Kit

INTENDED USE

Human SS-B Ab ELISA kit is designed for measuring in-vitro quantitative levels of IgG auto-antibodies to Sjögren syndrome type B (SS-B antibody, SS-B/La, SS-B Ab, anti-SSB) in human plasma or serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

BACKGROUND

Sjögren syndrome type B antigen (SS-B/La) are made up of 48-kD protein that is combined to RNA species and it is an extractable nuclear antigen (ENA). SS-B/La represent 1 of 4 autoantigens which includes SS-A/Ro, Sm and RNP. The following SS-B antibody ELISA kit can be useful tool for various diagnostic applications. Antibodies to ENAs are frequently found in patients with Sjogren’s syndrome, systemic rheumatic diseases, dermatomyositis/polymyositis, MCTD and systemic sclerosis. SS-B/La antibodies are predominately discovered in patients with Sjogren’s syndrome (approx. 60%) or lupus erythematosus (LE)(approx. 15%) and also the SS-B/La antibodies are infrequently found in the absence of SS-A/Ro antibodies.

SS-B AB ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified SS-B antibodies bound to microwells.
  • Calibrators (A-F): Concentration 0-200 U/ml, made up from anti SS-B antibody.
  • Controls Positive and Control Negative: Containing human SS-B antibody.
  • Sample Buffer P (5x).
  • Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
  • TMB Substrate.
  • Stop Solution: Contains acid.
  • Wash Buffer (50x Concentrated).
  • Instruction Manual.
  • Certificate of Analysis.

SENSITIVITY

The minimum detection sensitivity level of Sjögren syndrome type B (anti-SSB, SS-B Ab, SS-B/La) IgG autoantibodies using current human SS-B antibody ELISA kit was 1.0 U/ml. The dynamic range for this assay is 12.5 – 200.0 U/ml.

REFERENCES

  1. Antibodies against SS-B/La and SS-A/Ro antigens in patients with primary Sjögren’s syndrome. Scand J Rheumatol Suppl. (1986) 61: 93-7. Hansen B. and Manthorpe R.
  2. Estimation of amounts of anti-La(SS-B) antibody directed against immunodominant epitopes of the La(SS-B) autoantigen. Clin Exp Immunol. (1991) 85 (3): 402-6. Gordon T.P., et al.
  3. SS-B/La antigen purification, and ELISA detection of anti-SS-B/La antibodies in sera from patients with inflammatory connective tissue diseases. Allergy. (1988) 43 (8): 577-85. Hansen B., et al.
  4. Autoepitopes reactive with anti-SS-B/La. J Autoimmun. (1989) 2 (4): 345-51. Whittingham S., et al.

ADDITIONAL INFORMATION

  • Full Name: SS-B Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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