sPECAM-1 ELISA Kit (Soluble Platelet/Endothelial Cell Adhesion Molecule-1)

INTENDED USE

Human sPECAM-1 ELISA kit is a method for measuring in vitro quantitative levels of soluble platelet/endothelial cell adhesion molecule-1 (sPECAM1, CD31, sPECAM-1) in human serum, cell lysates, plasma, biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 0.22 ng/ml.

BACKGROUND

sPECAM1 or CD31 is a member of the immunoglobulin gene superfamily and plays important roles in removing aged neutrophils from the body. It is found to be widely distributed on vascular system cells which are responsible for mediating cellular interactions using either heterophilic or homophilic adhesive mechanisms.

sPECAM1 is predominately found to be located on macrophages, platelets, granulocytes, megakaryocyte, osteoclasts and lymphocytes (including B cells, B cells or NK cells). There have been lots of findings to indicate that it may be involved in; angiogenesis, integrin activation and leukocyte transmigration.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti PECAM-1 antibody.
• Standards: Concentrations 0, 0.781, 1.562, 3.125, 6.25, 12.5, 25.0, 50.0 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of soluble platelet/endothelial cell adhesion molecule-1 (sPECAM-1, CD31, PECAM1) using current sPECAM-1 ELISA kit was 0.469 ng/ml. The dynamic range for this assay is 0.781 – 50 ng/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PECAM-1, no cross reactivity or interference between PECAM-1and analogues was detected.
– Recovery: Serum (89 – 99%), EDTA Plasma (86 – 105%), Heparin Plasma (87 – 101%).
– Linearity: Serum (87 – 104%), EDTA Plasma (87 – 100%), Heparin Plasma (81 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Serum sPECAM-1 and sVCAM-1 levels are associated with conversion to multiple sclerosis in patients with optic neuritis. J Neuroimmunol. (2016) 300: 11-14. Kalinowska-Łyszczarz A., et al.
2. Dimer conformation of soluble PECAM-1, an endothelial marker. Int J Biochem Cell Biol. (2016) 77 (Pt A): 102-8. Jiang L., et al.
3. PECAM-1, a key player in neuroinflammation. Eur J Neurol. (2006) 13 (12): 1284-90. Review. Kalinowska A. and Losy J.
4. sPECAM-1 in serum and CSF of acute ischaemic stroke patients. Acta Neurol Scand. (2002) 106 (5): 292-8. Zaremba J. and Losy J.

ADDITIONAL INFORMATION

• Full Name: sPECAM-1 ELISA Kit (Soluble Platelet/Endothelial Cell Adhesion Molecule-1)
• Reactivity: Human
• Sample Type: Serum, Plasma, Other Biological Fluids, Tissue Homogenates
• Sensitivity: 0.469 ng/ml

OTHER RELATED ELISA KITS

• sICAM-1 ELISA Kit
• VCAM-1 ELISA Kit
• VE-Cadherin ELISA Kit