GET A QUOTE

Sesame ELISA Kit

  • Created on the 13 October, 2017.

BACKGROUND

Sesame is a flowering plant from the genus Sesamum (also called benne) which belongs to the family of Pedaliaceae. It has one of the highest oil content when compared to any other seeds and with its nutty, rich flavour is a common ingredient in cuisines throughout the world, from baked goods to sushi. Similar to many other foods and nuts, sesame can also cause an allergic reaction in some individuals. The fraction of proteins (approx. 16-32%) in sesame seed is relatively high and it is these proteins which are known for being allergic. The sensitivity to sesame can vary from one person to another, the reactions can also be unpredictable, even a tiny quantity can lead to an allergic reaction (severe cases leading to anaphylactic shock). For a person who is allergic to sesame, the hidden allergens in food can cause many critical problems since it is used in a large number of food preparations. To be sure of preventing a reaction, it is critical to avoid consuming sesame containing foods, researcher have highlighted sesame allergy as being a serious, significant and a growing issue since it is increasingly used in our diets. This ELISA assay represents a highly specific and sensitive protocol for measuring quantitative amounts of sesame residues in sauces, sausage, bakery products, soups and ice cream.

INTENDED USE

Sesame ELISA kit is a procedure designed for detecting quantitative concentrations of sesame residues in food (such as bakery products, ice cream, soups, sauces and sausage). This assay has a minimum analytical sensitivity limit of 0.2 ppm.

SENSITIVITY

The minimum detection sensitivity level of sesame residues using this sesame ELISA kit was 0.2 ppm. The standard assay range for this kit is 2.0 – 30.0 ppm.

REFERENCES

  1. Development and validation of a sandwich ELISA for the determination of potentially allergenic sesame (Sesamum indicum) in food. Anal Bioanal Chem. (2010) 398 (4): 1735-45. Redl G., et al.
  2. Identification of oleosins as major allergens in sesame seed allergic patients. Allergy. (2006) 61 (3): 349-56. Leduc V., et al.
  3. Analysis of glabrous canary seeds by ELISA, mass spectrometry, and Western blotting for the absence of cross-reactivity with major plant food allergens. J Agric Food Chem. (2013) 61 (25): 6102-12. Boye J.I., et al.
  4. Identification of oleosins as major allergens in sesame seed allergic patients. Allergy. (2006) 61 (3): 349-56. Leduc V., et al.
  5. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. Biomed Res Int. (2015) 2015: 853836. Koppelman S.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Sesame ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.2 ppm

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford