Serotonin ELISA Kit (5-Hydroxytryptamine)

INTENDED USE

Human serotonin ELISA kit is designed for determining in vitro quantitative concentrations of serotonin (5-hydroxytryptamine) using human serum, plasma, urine and platelets samples. This assay has a minimum detection limit of 6.2 ng/ml.

BACKGROUND

Serotonin (also known as 5-hydroxytryptamine); is found in the enterochromaffin cells. It functions in the regulation of gut movement. It is also important for cognitive functions such as; learning and memory. The modulation at synapses junction are thought to be a major action of many classes of pharmacological antidepressants. It is mainly metabolised to 5-HIAA and this is chiefly carried out by the liver. Metabolism involves an initial oxidation step by monoamine oxidase in order to form the corresponding aldehyde. This is followed by oxidation of aldehyde dehydrogenase to 5-HIAA, the indole acetic acid derivative. The latter is predominately excreted by the kidneys.

Serotonin is an example of an inhibitory neurotransmitter and medications that are used to increase the availability and uptake are vital in treating depression. It can be taken up by platelets, which are able to store it. When the platelets attach to a blood clot, this triggers the release serotonin which is vital in acting as a vasoconstrictor and a clotting factor to promote healing.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Serotonin Microtiter Strips: Antigen pre-coated microwell plate.
• Standards (A-F): Concentrations 0, 5, 15, 50, 150, 500, 2,500 ng/ml
• Antiserum: Rabbit anti-serotonin antibody.
• Control 1.
• Control 2.
• Enzyme Conjugate.
• Substrate: Chromogenic Tetramethylbenzidine (TMB).
• Wash Buffer Concentrate (50x).
• Stop Solution.
• Reaction Tubes.
• Acylation Reagent.
• Acylation Buffer.

SENSITIVITY

The minimum sensitivity level of 5-hydroxytryptamine (serotonin) using current serotonin ELISA kit was approximately 6.2 ng/ml. The dynamic range for this assay is 15 – 2,500 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: Limit of Detection (LOD) 6.2 ng/ml, Limit of Quantitation (LOQ) 10.2 ng/ml.
– Analytical Sensitivity (Cross Reactivity): Serotonin (100%), Melatonin (0.08%), Tryptamine (0.05%), Histidine (< 0.019%), Tyramine (< 0.018%), 5-Hydroxytryptophane, Phenylalanine, 5- Hydroxyindole acetic acid (< 0.014%).
– Precision Intra Assay: 9.2 – 13.8% (Urine) and 9.7 – 12.6% (Serum)
– Precision Inter Assay: 11.3 – 14.9% (Urine) and 10.4 – 12.4% (Serum)
– Linearity: 88 – 118% (Urine) and 18 – 113% (Serum)
– Recovery: 74 – 105% (Urine) and 89 – 126% (Serum)

REFERENCES

1. The role of serotonin and its receptors in activation of immune responses and inflammation. Acta Physiol (Oxf). (2015) 213 (3): 561-74. Review. Shajib M.S. and Khan W.I.
2. Polymorphisms of serotonin-neurotransmission and their effects on antipsychotic drug action. Pharmacogenomics. (2014) 15 (12): 1599-609. Review. Tang H., et al.
3. Metabolic kinetics of 5-hydroxytryptamine and the research targets of functional gastrointestinal disorders. Dig Dis Sci. (2014) 59 (11): 2642-8. Review. Jing F. and Zhang J.
4. Exercise and sleep in aging: emphasis on serotonin. Pathol Biol (Paris). (2014) 62 (5): 276-83. Review. Melancon M.O., et al.

ADDITIONAL INFORMATION

• Full Name: Serotonin ELISA Kit (5-Hydroxytryptamine)
• Reactivity: Human
• Sample Type: Serum, Plasma, Platelets, Urine
• Sensitivity: 6.2 ng/ml

OTHER RELATED ELISA KITS

• Tryptophan ELISA Kit
• Glutamate ELISA Kit
• Histamine ELISA Kit