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Serotonin ELISA Kit

  • Created on the 4 March, 2017.

BACKGROUND

Serotonin (5-hydroxytryptamine), is a monomer biogenic amine neurotransmitter that is a product of tryptophan metabolism. The majority of serotonin which is present in humans is located in the enterochromaffin cells of the digestive tract (gastrointestinal tract), here it is responsible for regulating movement of the gut. The remainder of the body’s serotonin is synthesized by serotonergic neurons within the central nervous system, these are known to have a number of different functions such as regulating sleep, appetite and mood. Serotonin is also found to have some cognitive functions, such as learning and memory. The modulation of serotonin at synapses junction are thought to be a major action of many classes of pharmacological antidepressants.

Serotonin is mainly metabolised to 5-HIAA and this is chiefly carried out by the liver. Metabolism involves an initial oxidation step by monoamine oxidase in order to form the corresponding aldehyde. This is then followed by oxidation of aldehyde dehydrogenase to 5-HIAA, the indole acetic acid derivative. The latter is predominately excreted by the kidneys.

Serotonin is generally an example of an inhibitory neurotransmitter and medications that are used to increase the availability and uptake of serotonin are vital in treating depression. Serotonin can also be taken up by platelets, which are able to store this hormone. When the platelets attach to a blood clot, this triggers the release serotonin which is vital in acting as a vasoconstrictor and a clotting factor to promote healing.

INTENDED USE

Serotonin ELISA kit is designed for determining in vitro quantitative concentrations of serotonin (5-hydroxytryptamine) human serum, plasma, urine and platelets. This assay has a minimum detection limit of 6.2 ng/ml.

SENSITIVITY

The minimum sensitivity level of serotonin (5-hydroxytryptamine) using this human serotonin ELISA kit was approximately 6.2 ng/ml. The dynamic assay range for this kit is 15 – 2,500 ng/ml.

REFERENCES

  1. The role of serotonin and its receptors in activation of immune responses and inflammation. Acta Physiol (Oxf). (2015) 213 (3): 561-74. Review. Shajib M.S. and Khan W.I.
  2. Polymorphisms of serotonin neurotransmission and their effects on antipsychotic drug action. Pharmacogenomics. (2014) 15 (12): 1599-609. Review. Tang H., et al.
  3. Metabolic kinetics of 5-hydroxytryptamine and the research targets of functional gastrointestinal disorders. Dig Dis Sci. (2014) 59 (11): 2642-8. Review. Jing F. and Zhang J.
  4. Exercise and sleep in aging: emphasis on serotonin. Pathol Biol (Paris). (2014) 62 (5): 276-83. Review. Melancon M.O., et al.
  5. Serotonin and beyond: therapeutics for major depression. Philos Trans R Soc Lond B Biol Sci. (2013) 368 (1615): 20120536. Review. Blier P. and El Mansari M.

ADDITIONAL INFORMATION

  • Full Name: Serotonin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Platelets, Urine
  • Sensitivity: 6.2 ng/ml

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