GET A QUOTE

RNP/Sm Ab ELISA Kit

  • Created on the 29 June, 2017.

BACKGROUND

A selection of antinuclear antibodies such as ribonucleoprotein antibodies (RNP), anti-smith (anti-Sm) and anti-double-stranded deoxyribonucleic acid (dsDNA) are known to be highly specific in aiding the diagnosis of systemic lupus erythematosus (SLE). In the case of antibodies against U1 ribonucleoprotein (RNP) complex, these are vital markers for mixed connective tissue disease (MCTD), can also be found in patients having SLE and numerous other diseases such as scleroderma, polymyositis and Sjögren’s syndrome. Elevated levels of antibodies to RNP can be found in MCTD patients whereas in SLE patients RNP antibodies have been linked to relatively benign disease that have lower incidence of central nervous system and renal involvement. Patients are generally considered positive for RNP antibodies RNP antibody result is significantly greater than that of the Sm antibody result.

INTENDED USE

Human RNP/Sm ELISA kit can be used for detecting in-vitro quantitative amounts of IgG autoantibodies to ribonucleoprotein and Anti-Smith (RNP/Sm) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human ribonucleoprotein and Anti-Smith IgG autoantibodies using this human RNP/Sm ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 12.5 – 200.0 U/ml.

REFERENCES

  1. An immunoassay differentiating sera with antibodies to Sm alone, antibodies to Sm/RNP complex, and antibodies to RNP alone. Arthritis Rheum. (1987) 30 (4): 389-96. Tsay G.J., et al.
  2. The relationship between Sm and RNP antigens. Clin Immunol Immunopathol. (1986) 41 (1): 91-107. Fronek Z., et al.
  3. Anti-Sm and anti-RNP antibodies. Autoimmunity. (2005) 38 (1): 47-54. Review. Migliorini P., et al.
  4. Two major autoantibody clusters in systemic lupus erythematosus. PLoS One. (2012) 7 (2): e32001. Ching K.H., et al.
  5. Contrasting molecular patterns of MHC class II alleles associated with the anti-Sm and anti-RNP precipitin autoantibodies in systemic lupus erythematosus. Arthritis Rheum. (1993) 36 (1): 94-104. Olsen M.L., et al.
  6. Antibodies to Sm and RNP. Prognosticators of disease involvement. Arthritis Rheum. (1983) 26 (7): 848-53. Munves E.F. and Schur P.H.

ADDITIONAL INFORMATION

  • Full Name: RNP/Sm Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.0 U/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford