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Respiratory Syncytial Virus (RSV) IgA ELISA Kit

  • Created on the 8 September, 2017.

BACKGROUND

Respiratory-syncytial virus (RSV) is a syncytial virus that is responsible for causing infections of the respiratory tract and lungs. It is a single stranded, negative sense RNA virus which belongs to the Pneumoviridae family. Human RSV is an approx. 120–200 nm enveloped virus which contains a linear negative-sense RNA genome and a lipoprotein coat. There are four viral genes which code for intracellular proteins that are involved in processes such as replication, transcription and particle budding. These are called matrix protein (M), “large” protein (L), Nucleoprotein (N) and phosphoprotein (P). The genome itself is about 15,000 nucleotides in length and is made up of single stranded RNA (displaying negative polarity) and it contains 10 genes in total which encode 11 proteins. RSV is mainly transmitted through large droplets and also by secretions from contact with any infected people. The virus has an incubation period of 3-5 days before the symptoms begin to appear. ELISA assay have played important role for the serological diagnosis of RSV infections because of their sensitivities, specificities and the ability to allow differentiation of antigens into the various immunoglobulin classes.

INTENDED USE

Human RSV IgA ELISA kit is intended for determining in-vitro quantitative levels of human IgA antibodies against respiratory syncytial virus (RSV) in serum or plasma. This assay has a minimum analytical sensitivity limit of 1.7 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human respiratory syncytial virus IgA using this human RSV IgA ELISA kit was 1.7 U/ml. The dynamic assay range for this kit is 1.0 – 100.0 U/ml.

REFERENCES

  1. Association between pronounced IgA response in RSV bronchiolitis and development of allergic sensitization. Pediatr Allergy Immunol. (1997) 8 (1): 1-6. Strannegård O., et al.
  2. Vaccination with the RSV fusion protein formulated with a combination adjuvant induces long-lasting protective immunity. J Gen Virol. (2014) 95 (Pt 5): 1043-54. Garg R., et al.
  3. A single intranasal immunization with a subunit vaccine formulation induces higher mucosal IgA production than live respiratory-syncytial virus. Virology. (2016) 499: 288-297. Garg R., et al.
  4. Immunoglobulin A antibody response to respiratory-syncytial virus structural proteins in colostrum and milk. J Clin Microbiol. (1989) 27 (9): 1949-51. Tsutsumi H., et al.

ADDITIONAL INFORMATION

  • Full Name: Respiratory Syncytial Virus (RSV) IgA ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.7 U/ml

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