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Resistin ELISA Kit

INTENDED USE

Human resistin ELISA kit is a reliable quantitative procedure for measuring resistin in human serum, plasma, saliva, breast milk, cell culture medium and urine. This assay has a minimum sensitivity detection limit of 0.012 ng/ml.

BACKGROUND

Resistin is a cysteine-rich protein that is 11.3 kDa and belongs to the protein family of resistin-like molecules (RELM) which also includes: RELMa, RELMb and RELMg. It was first identified in mice, in humans no other RELM family are found apart from resistin and RELMb. There is a 53% homology between the human and murine protein, both containing 11 cysteine-residues, synthesized as a propetide of 108 amino acids that is secreted as a dimer which is linked together by a disulfide bridge of cysteine residues. The structure of human resistin is highly dependent on its concentration, there is evidence to suggest there is a concentration dependent shift from a-helical to b-sheet structure. The standard that is used in the following assay t has a molecular mass between 51-53KDa and it has the ability to detect resistin including the active enzyme and zymogen.

Resistin expression has be identified in white adipose tissue, pancreatic islets and pituitary of mice. In rats it can also be found in brown adipose tissues, whereas in humans the expression in adipocytes can only be detected at very low levels. However, the resistin gene can also be expressed in bone marrow, pre-adipocytes marcophages and pancreatic islets. Resistin is an adipose derived hormone that is postulated to link adiposity to insulin resistance. Furthermore, type 2 diabetes has been characterized by a specific target-tissue resistance to insulin which is strongly associated to obesity and the epidemic is most commonly found in industrialised societies.

RESISTIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Break apart strips, coated with human resistin antibody.
  • Standards A-E: Lyophilised recombinant human resistin. Concentration 0.02, 0.10, 0.30, 0.60, 1.00 ng/ml.
  • Sample Buffer.
  • Dilution Buffer.
  • Control Sera KS1 and Control Sera KS2.
  • Antibody Conjugate: Contains biotinylated anti-resistin antibody.
  • Enzyme Conjugate: Consisting of horseradish peroxidase (HRP) labelled streptavidin.
  • Washing Buffer Solution (20x Concentrated).
  • TMB Substrate.
  • Stopping Solution: 0.4 N acidic solution.
  • Sealing Tape: To cover microtiter plate.

SENSITIVITY

The minimum sensitivity detection limit of human resistin using current resistin ELISA kit was approximately 0.012 ng/ml. The dynamic range for this assay is 0.02 – 1.0 ng/ml.

ASSAY CHARACTERISTICS

– Reisstin: ELISA
– Analytical Sensitivity: 0.012 ng/ml (12 pg/ml)
– Species Specificity: No cross reactivity detected in the following species: horse, donkey, sheep, bovine, pig, rabbit, goat, rat, chicken, dog, mouse, cat, guinea pig
– Interference: None
– Intra-Assay Variability: 4.49 – 4.97%
– Inter-Assay Variability: 3.37 – 6.67%
– Recovery And Linearity: 92.7 – 103.4%

REFERENCES

  1. The hormone resistin links obesity to diabetes. Nature (2001) 409: 307-312. Steppan, C. M., et al.
  2. Resistin: a potential biomarker for periodontitis influenced diabetes mellitus and diabetes induced periodontitis. Dis Markers. (2014) 2014: 930206. Review. Devanoorkar A., et al.
  3. Resistin: insulin resistance to malignancy. Clin Chim Acta. (2015) 438: 46-54. Review. Codoñer-Franch P. and Alonso-Iglesias E.
  4. Resistin induces lipolysis and suppresses adiponectin secretion in cultured human visceral adipose tissue. Regul Pept. (2014) 194-195: 49-54. Chen N., et al.

ADDITIONAL INFORMATION

  • Full Name: Resistin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Breast Milk, Saliva, Urine, Cell Culture
  • Sensitivity: 0.012 ng/ml

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