- Created on the 12 February, 2017.
Human resistin ELISA kit is a reliable quantitative procedure for measuring resistin in human serum, plasma, saliva, breast milk, cell culture medium and urine. This assay has a minimum sensitivity detection limit of 0.006 ng/ml.
Resistin is a cysteine-rich protein that is 11.3 kDa and belongs to the protein family of resistin-like molecules (RELM) which also includes: RELMa, RELMb and RELMg. It was first identified in mice, in humans no other RELM family are found apart from resistin and RELMb. There is a 53% homology between the human and murine protein, both containing 11 cysteine-residues, synthesized as a propetide of 108 amino acids that is secreted as a dimer which is linked together by a disulfide bridge of cysteine residues. The structure of resistin is highly dependent on its concentration, there is evidence to suggest there is a concentration dependent shift from a-helical to b-sheet structure.
Resistin expression has be identified in white adipose tissue, pancreatic islets and pituitary of mice. In rats it can also be found in brown adipose tissues, whereas in humans the expression in adipocytes can only be detected at very low levels. However, the human resistin gene can also be expressed in bone marrow, pre-adipocytes marcophages and pancreatic islets. Resistin is an adipose derived hormone that is postulated to link adiposity to insulin resistance. Furthermore, type 2 diabetes has been characterized by a specific target-tissue resistance to insulin which is strongly associated to obesity and the epidemic is most commonly found in industrialised societies. The standard that is used in the following procedure has a molecular mass between 51-53KDa and it has the ability to detect human resistin including the active enzyme and zymogen.
Reagents which are supplied with the following resistin ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: coated with human resistin antibody.
2. Standards (A-E): values of 20, 100, 300, 600 und 1000 pg/ml.
3. Sample Buffer.
4. Dilution Buffer.
5. Control Sera KS1 and KS2.
6. Antibody Conjugate.
7. Enzyme Conjugate.
8. Washing Buffer.
10. Stopping Solution.
11. Sealing Tape.
The minimum sensitivity detection limit of resistin using current human resistin ELISA kit was approximately 0.06 ng/ml. The dynamic range for this assay is 0.02 – 1.0 ng/ml.
- The hormone resistin links obesity to diabetes. Nature (2001) 409: 307-312. Steppan, C. M., et al.
- Resistin: a potential biomarker for periodontitis influenced diabetes mellitus and diabetes induced periodontitis. Dis Markers. (2014) 2014: 930206. Review. Devanoorkar A., et al.
- Resistin: insulin resistance to malignancy. Clin Chim Acta. (2015) 438: 46-54. Review. Codoñer-Franch P. and Alonso-Iglesias E.
- Resistin induces lipolysis and suppresses adiponectin secretion in cultured human visceral adipose tissue. Regul Pept. (2014) 194-195: 49-54. Chen N., et al.
- Full Name: Resistin ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Breast Milk, Saliva, Urine, Cell Culture Medium
- Sensitivity: 0.06 ng/ml