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Renin (Active) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Renin is a polypeptide enzyme with has a molecular weight of 37kDa, it is also known as angiotensinogenase and belongs to the aspartic acid protease family. Renin is a member of Renin-Angiotensin-Aldosterone System (RAAS) that is responsible for regulating and controlling blood pressure, sodium/potassium homeostasis, renal blood flux and glomerular filtration. Essentially a protease that is secreted by juxtaglomerular cells of the kidneys when the body is low in NaCl content and blood volume.

Plasmatic concentration of renin are found to be influenced by the concentration of circulating angiotensinogen and angiotensin II, where renin secretion can be reduced if there are high plasmatic levels of angiotensin II present. This property makes the measurement of renin plasma concentrations useful for the diagnosis of hypertension and for checking hypertensive patients following therapeutic treatments. It is a good index for measuring RAAS activity and in case of any dysfunction, this renin ELISA will allow clinical implications for diagnosis, treatment and any subsequent follow-up. Plasma renin is a good index for the activity of the RAAS. In case of dysfunction of the RAAS, the Renin assay will allow clinical implications for diagnosis, treatment, and follow up. Active renin should be used to measure: differential diagnosis of hyperaldosteronism, diagnosis of hypertension, differential diagnosis of hypokalemia, diagnosis of isolated deficit in mineral corticoids, diagnosis of insufficient response to antihypertensive treatment, detection of renin producing tumors in the kidney and monitoring of glucocorticoid therapy.

INTENDED USE

Renin (active) ELISA kit is a reliable quantitative procedure for measuring direct active renin present in human serum and plasma (heparin and EDTA). This assay has a sensitivity detection limit of 0.81 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of active renin using this direct human renin active ELISA kit was approximately 0.81 pg/ml. The dynamic assay range for this kit is 4.0 – 128.0 pg/ml.

REFERENCES

  1. Proteolytic processing of human prorenin in renal and nonrenal tissues. Kidney Int. (1994) 46, 1522–1524. Reudelhuber T.L., et al.
  2. Angiotensin AT2 receptors: control of renal sodium excretion and blood pressure. Trends Endocrinol Metab. (2008) 19, 84-7. Carey R.M. and Padia S.H.
  3. Specimen processing and renin activity in plasma. Clin. Chem. (1992) 38, 598. Nicar M.J.
  4. Pivotal role of the renin/prorenin receptor in angiotensin II production and cellular responses to renin. J Clin Invest. (2002) 109, 1417–1427. Nguyen G., et al.

ADDITIONAL INFORMATION

  • Full Name: Renin (Active) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA, Heparin)
  • Sensitivity: 0.81 pg/ml

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