- Created on the 18 February, 2017.
Progesterone ELISA kit is a reliable quantitative procedure for measuring progesterone (pregn-4-ene-3 or 20-dione) in human serum and plasma samples. This assay has a minimum sensitivity detection limit of 0.045 ng/ml.
Progesterone (also referred to as pregn-4-ene-3 or 20-dione) is a C21 steroid hormone which consists of a keto-group at the C3 position and a double bond between the C4 and C5. It has molecular weight of 314.5 Da and it can be found to be synthesised from cholesterol through pregnenolone within the granulosa and theca cells of the corpus iuteum that are under the influence of Luteinizing hormone (LH). Progesterone belongs to the group of neuro-steroids which can be found in high concentrations in certain areas in the brain and can also be are synthesised there. Neuro-steroids have the ability to affect synaptic functioning, myelinisation and have neuroprotective activity.
Progesterone is essentially a female sex hormone which can act in conjunction with estrogens to prepare endometrium for the implantation of the blastocyte, regulate accessory organs during menstrual cycle and is vital in the generally maintaining pregnancy. During pregnancy the placenta is the major source of progesterone, whereas in non-pregnant women it is mainly secreted by the corpus luteum. The maximal levels is achieved between days 4-7 after ovulation and this can be found to remain elevated for an additional 4-6 prior to falling back to the pre-ovulatory levels 24 hours before the onset of menstruation. Due to the fact that level of increase and fall of progesterone parallels the activity of ovarian follicle and corpus luteum, the determination of plasma progesterone levels have been clinically useful in confirming ovulation and the normal functioning of the corpus luteum for non- pregnant women. There have evidence for abnormal progesterone secretion which has been implicated in dysmenorrhoea, premenstrual tension, luteal insufficiency and irregular shedding of endometrium.
The cuurent assay procedure measures the total (unconjugated) progesterone in serum. We also have available an assay for measuring free progesterone in saliva.
Reagents which are supplied with the following progesterone ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: polyclonal anti progesterone antibody coated.
2. Standard (Standard 0-6): 0; 0.3; 1.25; 2.5; 5; 15; 40 ng/mL.
3. Progesterone conjugated to horseradish Peroxidase.
4. Substrate Solution.
5. Stop Solution.
6. Wash Solution (40X).
The minimum sensitivity detection limit of pregn-4-ene-3 (20-dione) using current human progesterone ELISA kit was approximately 0.045 ng/ml. The dynamic range for this assay is 0.3 – 40.0 ng/ml.
- Clinical use of progesterone in infertility and assisted reproduction. Acta Obstet Gynecol Scand. (2015) 94 Suppl 161: 17-27. Review. Ciampaglia W. and Cognigni G.E.
- Physiology, production and action of progesterone. Acta Obstet Gynecol Scand. (2015) 94 Suppl 161: 8-16. Review. Taraborrelli S.
- The role of progesterone in memory: an overview of three decades. Neurosci Biobehav Rev. (2015) 49: 193-204. Review. Barros L.A., et al.
- Progesterone-Regulated Endometrial Factors Controlling Implantation. Am J Reprod Immunol. (2016) 75 (3): 237-45. Review. Bhurke A.S., et al.
- Full Name: Progesterone ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 0.045 ng/ml