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Plasma Renin Activity (PRA) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Renin which can also be called angiotensinogenase is a 37kDa polypeptide enzyme that is a member of the aspartic acid protease family. The determination of plasma renin activity (PRA) is a vital tool in clinical evaluation of hypertensive patients, where it can assist in diagnosing primary hyperaldosteronism and provide help during the therapy treatment of other forms of hypertension.

In contrast to measuring renin concentrations, the determination of PRA is found to be a more accurate indicator of hyperaldosteronism (PHA) because of many reasons for example. Plasma renin concentration assay is unable to ensure sensitivity in low renin states, whilst the assay sensitivity can be enhanced through increasing incubation step during the generation step. PRA is used to express the rate of Ang-I formation by action of renin on its substrate angiotensinogen, this makes PRA dependent on concentrations of both renin and angiotensinogen. Whereas the concentrations of angiotensinogen is ignored in renin concentration assays. This ELISA assay measures PRA concentrations and the results are expressed in terms of mass of angiotensin-I (Ang-I) that are generated per volume of human plasma in unit time (ng/mL.h).

INTENDED USE

Plasma renin activity ELISA kit is a reliable quantitative procedure for measuring renin activity that is present in plasma. This assay has a sensitivity detection limit of 0.14 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of human plasma renin using this plasma renin activity ELISA kit was approximately 0.14 pg/ml. The dynamic assay range for this kit is 0.2 – 60.0 pg/ml.

REFERENCES

  1. Plasma renin activity (PRA) levels and antihypertensive drug use in a large healthcare system. Am J Hypertens. (2012) 25 (3): 379-88. Sim J.J., et al.
  2. Renin-angiotensin system blockers may create more risk than reward for sodium-depleted cardiovascular patients with high plasma renin levels. Am J Hypertens. (2013) 26 (6): 727-38. Review. Sealey J.E., et al.
  3. Plasma renin testing to guide antihypertensive therapy. Curr Hypertens Rep. (2015) 17 (1): 506. Review. Viera A.J and Furberg C.D.
  4. Cost-effectiveness of renin-guided treatment of hypertension. Am J Hypertens. (2013) 26 (11): 1303-10. Smith S.M and Campbell J.D.

ADDITIONAL INFORMATION

  • Full Name: Plasma Renin Activity (PRA) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma
  • Sensitivity: 0.14 pg/ml

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