Pecan Nut ELISA Kit

Full Name: Pecan Nut ELISA Kit
Sample Type: Food (Sausage, Ice, Cream, Cereals, Chocolate)
Sensitivity: 0.2 ppm

BACKGROUND

Pecan (also known as Carya illinoinensis) is a nut which comes from a tree that belongs to the walnut family (called Juglandiaceae). It is a species of the hickory native to Mexico and southern regions of United States. The amount of protein which is contained in a pecan nut is approx. 9% and some of these proteins are found to cause allergic reactions (for example the 11S legumin Car I 4 and the 2S albumin Car I 1).

The symptoms of pecan allergy vary considerably from one individual to another and this can also fluctuate from being mild to life-threatening in a single a single person based other specific factors and how much is consumed. Usually, the symptoms occur quickly within one hour of eating a pecan or a pecan-containing substance.

One of the most effective way in preventing an allergic reaction is to avoid pecan exposure. This is quite difficult since very low levels of pecan can trigger an allergic reaction and in some severe cases lead to anaphylactic shock. Also, the existence of cross-contamination, is often noticed that is frequently due to a number of production process.

INTENDED USE

Pecan nut ELISA kit is intended for detecting quantitative levels of pecan nut residues in food (for example sausage, cereals, ice cream and chocolate). This assay has a minimum analytical sensitivity limit of 0.2 ppm.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Plate: Coated with anti pecan nut antibodies.
Pecan nut Standards 1-5: Concentration 0, 2, 6, 20, 60 ppm.
Conjugate (Anti-Pecan-Nut-Peroxidase).
Substrate Solution (TMB).
Stop Solution (0.5 M H2SO4).
Extraction and sample dilution buffer (TRIS)(10x).
Washing Solution (PBS + Tween 20)(10x Concentrate).
Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of pecan nut residues using current pecan nut ELISA kit was 0.2 ppm. The standard range for this assay is 2.0 – 60.0 ppm.

ASSAY CHARACTERISTICS

– Sensitivity: Limit of detection, LOD (0.2ppm), Limit of quantification, LOQ (2.0ppm)
– Intra-Assay Precision: 8%
– Inter-Assay Precision: 6 – 11%
– Linearity: 86 – 96%
– Specificity (Cross Reactivity): Walnut (0.0035%), Carrot (0.0006%), Hazelnut, Macadamia nut (0.0003%). No cross reaction was detected for the following: Almond, Chestnut, Egg white powder, Onion, Rapeseed, Apricot, Chicken, Ewe’s milk, Paprika, Rice, Barley, Chickpea, Fish gelatine, Pea, Rye, Bean (white), Cocoa, Gliadin, Peach, Saccharose, Beef, Coconut, Goat’s milk, Peanut, Sesame, Bovine gelatine, Cod, Guar gum, Pepper, Shrimp (cooked), Brazil nut, Corn, Kiwi, Pine seed, Shrimp (raw), Buckwheat, Cow’ milk, Lamb, Pistachio, Soy, Carob gum, Crab (cooked), Lentil, Plum, Soy lecithin, Cashew, Crab (raw), Lupin, Poppy seed, Sunfl. Seeds, Cayenne, Cress, Mustard, Pork, Tofu, Celery, Cumin, Nutmeg, Potato, Tomato, Cherry, Egg, Oats, Pumpkin seed, Wheat.
– Recovery: Cornflakes (114%), Cookies (107%), Ice-cream (93%), Sausage (92%), Chocolate (78%).

REFERENCES

The potential of the pecan-nut cake as an ingredient for the food industry. Food Res Int. (2020) 127: 108718. Maciel L.G. et al.
Hepatoprotective effects of pecan nut shells on ethanol-induced liver damage. Exp Toxicol Pathol. 2013 Jan;65(1-2):165-71. Müller L.G. et al.
Application of Multiantigen Profiling To Detect Pecan. J Food Prot. (2018) 81 (5): 700-704. Cho CY et al.
Appetite responses to pecan-enriched diets. Appetite. (2022) 173: 106003. Guarneiri LL et al.

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