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Peanut ELISA Kit

  • Created on the 12 October, 2017.

BACKGROUND

Peanut (also called goober and groundnut), it is a legume crop which is classified as Arachis hypogaea. The fraction of proteins (approx. 25%) is relatively high in peanuts and it is these proteins that are found to cause allergic reactions (proteins like conarachins and arachins). Some people have reported from mild to severe experiences of allergic reactions to exposure to peanuts. The symptoms can range anything from watery eyes to fatal anaphylactic shock, if left untreated. For these people even consuming a small quantity of peanut can lead a reaction. Cross-contamination, which usually occurs during many production process is also often noticed (for example during the production of chocolate). The ELISA assay offers a highly sensitive method for quantifying concentrations of peanut residues present in foods like chocolate, cereals, cookies and ice cream.

INTENDED USE

Peanut ELISA kit can be used for measuring quantitative amounts of peanut residues in food (such as cookies, chocolate, ice cream and cereals). This assay has a minimum analytical sensitivity limit of 0.1 ppm.

SENSITIVITY

The minimum detection sensitivity level of peanut residues using this peanut ELISA kit was 0.1 ppm. The standard assay range for this kit is 1.0 – 40.0 ppm.

REFERENCES

  1. ELISA kit for peanut protein determination: collaborative study. J AOAC Int. (2013) 96 (5): 1041-7. Lexmaulová H., et al.
  2. Impact of thermal processing on ELISA detection of peanut allergens. J Agric Food Chem. (2013) 61 (24): 5649-58. Fu T.J. and Maks N.
  3. Immune reactivities to peanut proteins, agglutinins, and oleosins. Altern Ther Health Med. (2015) 21 Suppl 1: 73-9. Vojdani A.
  4. Effect of processing on the detectability of peanut protein by ELISA. Food Chem. (2013) 141 (3): 1651-4. Iqbal A. and Ateeq N.
  5. Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. J Agric Food Chem. (2015) 63 (6): 1849-55. Jayasena S., et al.

ADDITIONAL INFORMATION

  • Full Name: Peanut ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.1 ppm

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Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford