- Created on the 1 February, 2017.
Human PTH intact ELISA kit is a reliable quantitative method for measuring intact parathormone (intact-PTH or parathyrin) in human serum samples. This assay has a sensitivity detection limit of 1.72 pg/ml.
Parathyroid hormone (PTH) that is also referred to either parathormone or parathyrin is a molecular precursor that is made up of 115 amino acids and is biosynthesised as a pre-proparathyroid hormone in the parathyroid gland. Following sequential intracellular cleavage and proteolytic modification the proparathyroid hormone is eventually converted to an 84 amino acid polypeptide which is parathyroid hormone.
The regulation of PTH secretion in healthy individuals occurs through a negative feedback mechanism of serum calcium on the parathyroid glands. The biologically active form is represented by the intact PTH, it has a half-life of under four minutes and has the ability to clear rapidly from circulation. The measurement of intact PTH is vital for differentiation of primary hyperparathyroidism from other forms of hypercalcemia, for example thyrotoxicosis, sarcoidosis and malignancy. Parathyroid hormone levels have been found to be elevated in patients with primary hyperparathyroidism. This has made it a specific and helpful tool in diagnosis of primary hyperparathyroidism.
Reagents which are supplied with the following PTH intact ELISA kit, please following instructions for storage conditions.
1. Microtitre Plate: One holder with streptavidin coated strips.
2. Calibrators (A-F).
3. Controls (1+2).
4. Reagent 1: Biotinylated PTH antibody.
5. Reagent 2: Peroxidase labelled PTH antibody.
6. Reagent 3: Sample diluent.
7. Reagent 4: Reconstitution solution.
8. Reagent A: Wash Buffer.
9. Reagent B: TMB substrate.
10. Stopping Solution.
The minimum sensitivity detection limit of parathyroid hormone (intact PTH) using current human PTH intact ELISA kit was approximately 1.72 pg/ml. The dynamic range for this assay is 12.7 – 966 pg/ml.
- Intact PTH is stable in unfrozen EDTA plasma for 48 hours prior to laboratory Analysis. Clin. Chem. (1995) 41:6: page S47. Kruger, L., et al.
- Metabolism of parathyroid hormone: physiological and clinical significance. Am. J. Med. (1974) 56: 774. Segre, G.V., et al.
- The parathyroid polyhormones: New concepts in the spectrum of peptide hormone action. Endocrin. Rev. (1991) 12:110-117. Mallette, L.E.
- Comparison of commercially available parathyroid hormone immunoassay in the differential diagnosis of hypercalcemia due to primary hyperparathyroidism or malignancy. Ann. Intern. Med. (1979) 91:739-740. Raisz, L.G., Yajnik, C.H., Bockman, R.S., and Bower, B.B.
- Clinical performance of parathyroid hormone immunometric assays. Mayo Clin. Proc. (1992) 67:637-645. Kao, P.C., van Heerden, J.A., Grant, C.S., Klee, G.G., Khosla S.
- Full Name: Parathyroid Hormone (PTH) Intact ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 1.72 pg/ml