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Osteopontin ELISA Kit (Rat)

  • Created on the 20 August, 2018.

INTENDED USE

Rat osteopontin ELISA kit is intended for measuring quantitative concentrations of rat osteopontin (OPN) using EDTA plasma, cell culture media or urine samples. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.

BACKGROUND

Osteopontin (OPN) is a glycosylated highly acidic calcium binding phosphoprotein which is released by vascular smooth muscle cells, kidney tubule cells, osteoblasts and activated T-cells. Due to glycosylation and phosphorylation the molecular weight of OPN can range from 44-66 kDa. The amino acid sequence Arg-Gly-Asp (RGD) is present and different forms of osteopontin can be found to be present because of RNA splicing, glycosylation, sulfation and phosphorylation. Both OPN and thrombin localise together at sites of injury, angiogenesis, inflammation and tumor cells.

SENSITIVITY

The minimum sensitivity detection limit of OPN using current rat osteopontin ELISA kit is 0.01 ng/ml. The dynamic range for this assay is 0.07 – 4.75 ng/ml.

REFERENCES

  1. Osteopontin synthesis and localization along the human nephron. J Am Soc Nephrol. (2002) 13 (5): 1210-8. Verhulst A., et al.
  2. High Concentration of Calcium Stimulates Calcium Oxalate Crystal Attachment to Rat Tubular Epithelial NRK Cells Through Osteopontin. Urology. (2015) 86 (4): 844. e1-5. Xi Q., et al.
  3. Influence of osteopontin short hairpin RNA on the proliferation and activity of rat vascular smooth muscle cells. J Huazhong Univ Sci Technolog Med Sci. (2009) 29 (2): 144-9. Ye S., et al.
  4. The role of transglutaminase 2 and osteopontin in matrix protein supplemented microencapsulation of marrow stromal cells. Biomaterials. (2010) 31 (35): 9256-65. Hakimzadeh N., et al.
  5. A combination of sinomenine and methotrexate reduces joint damage of collagen induced arthritis in rats by modulating osteoclast-related cytokines. Int Immunopharmacol. (2014) 18 (1): 135-41. Sun Y., et al.

ADDITIONAL INFORMATION

  • Full Name: Osteopontin ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Urine, EDTA Plasma, Cell Culture Media
  • Sensitivity: 0.01 ng/ml

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