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Osteocalcin ELISA Kit

INTENDED USE

Human osteocalcin ELISA kit is a reliable quantitative procedure for measuring osteocalcin (OST) in human serum. This assay has a sensitivity detection limit of 0.8 ng/ml.

BACKGROUND

Osteocalcin (OST) which is also called bone Gla protein (B.G.P) is a 49 amino-acids peptide which contains three residues of gamma carboxyl glutamic acids and is 5800 Da in molecular weight. It is synthesised in the bone by the osteoblasts where after its production it is partly incorporated in the bone matrix, the rest of it is found to be circulating in blood. There are a large number of publications which show a direct correlation between the rate of bone formation and the levels of circulating human osteocalcin but the exact physiological function is yet still unclear.

The following human osteocalcin ELISA kit is an essential tool for measuring many different diagnostic applications. In various clinical applications determining the levels of human osteocalcin is helpful in many cases for example when:
(a). monitoring bone metabolism during the perimenopause and post-menopause.
(b). monitoring bone metabolism in patients that have growth hormone deficiency, chronic renal failure, hypothyroidism and hyperthyroidism.
(c). identifying women that are at potential risk of developing osteoporosis.
(d). monitoring bone metabolism during treatment of premenopausal women with LH-RH agonists and hormone replacement therapy.

OSTEOCALCIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterplate: Coated anti human osteocalcin antigen.
  • Conjugate Buffer.
  • Conjugate: HRP labelled conjugated to anti human osteocalcin antibody.
  • Calibrators 1-5: Concentration 0-75 ng/ml. Made from anti human osteocalcin.
  • Wash Solution (200x Concentrated).
  • Controls 1 and 2: Containing anti human osteocalcin.
  • Chromogenic Solution: Tetramethylbenzydine (TMB).
  • Stop Solution: HCl (1N).

SENSITIVITY

The minimum sensitivity detection limit of osteocalcin (OST) using current human osteocalcin ELISA kit was approximately 0.8 ng/ml. The dynamic range for this assay is 1.9 – 90.0 ng/ml.

REFERENCES

  1. Osteocalcin: a potential marker of metastatic bone disease and response to treatment. Eur. J. Cancer Clin. Oncol.(1988) 24,1211-1217. R.E. Coleman, et al.
  2. Osteocalcin: Diagnostic Methods and Clinical Applications. Crit. Rev. Clin. Lab. Sci. (1991) 28, 4, 287-335. M.J. Power and P.F. Fottrell.
  3. An evaluation of serum osteocalcin in Paget’s disease of bone and its response to diphosphonate treatment. Arthritis and Rheumatism (1988) 31, 9, 1142-1147. L.A. Coulton, et al.
  4. Serum BGP: a specific marker for bone formation in postmenopausal osteoporosis. The Lancet (1984) 1091-1093. J.P. Brown et al.

ADDITIONAL INFORMATION

  • Full Name: Osteocalcin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.8 ng/ml

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