GET A QUOTE

Normetanephrine Urine ELISA Kit

  • Created on the 4 March, 2017.

BACKGROUND

Normetanephrine is a catecholamine metabolite of norepinephrine and it is formed through the action of catechol-O-methyl transferase on norepinephrine. Cells that are found to be derived from neuroendocrine tumors (for example pheochromcytoma) can produce catecholamines that are secreted episodically using vesicles into the blood stream. However, there are a small portion of the catecholamines which can be metabolised inside the cells into their corresponding catecholamines metabolites and these are namely metanephrine, normetanephrine and 3-methoxytyramine. These catecholamines can be continuously secreted at low levels into the blood stream.

In clinical medicine, these catecholamines have been useful in providing diagnostic tools for identifying patients that are suspected of having a tumor of chromaffin cells such as pheochromocytoma and paraganglioma. These metabolites (normetanephrine and metanephrine) can be measured in plasma or urine samples. The determination of conjugated normetanephrine in urine is a well-established and long-standing technique. Current literature is indicating that the analysis of urine normetanephrine is an accurate biochemical marker for identifying patients suspected of pheochromocytoma. The current guidelines recommend the use of either plasma free normetanephrines or urinary fractionated normetanephrines for the initial biochemical phase.

INTENDED USE

Normetanephrine ELISA kit is intended for analysing in vitro quantitative levels of normetanephrine (NM) in human urine samples. This assay has a minimum detection limit of 23 ng/ml.

SENSITIVITY

The minimum sensitivity level of normetanephrine (NM) in urine using this human normetanephrine ELISA kit was approximately 23 ng/ml. The dynamic assay range for this kit is 30 – 3,000 ng/ml.

REFERENCES

  1. Evaluation of a urinary metanephrines reagent kit: an automated approach. Singapore Med J. (2008) 49 (6): 454-7. Thevarajah M.T., et al.
  2. Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma. Scand J Clin Lab Invest. (2011) 71 (8): 695-700. Christensen T.T., et al.
  3. Catecholamine metabolites in urine, as chronic stress biomarkers, are associated with higher risk of chronic periodontitis in adults. J Periodontol. (2014) 85 (12): 1755-62. Mesa F., et al.
  4. Intraabdominal pulmonary sequestration presenting with elevated urinary normetanephrine levels. J Pediatr Surg. (2009) 44 (3): e11-4. Alaish S.M., et al.
  5. Laboratory evaluation of pheochromocytoma and paraganglioma. Clin Chem. (2014) 60 (12): 1486-99. Review. Eisenhofer G. and Peitzsch M.

ADDITIONAL INFORMATION

  • Full Name: Normetanephrine Urine ELISA Kit
  • Reactivity: Human
  • Sample Type: Urine
  • Sensitivity: 23 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford