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Noradrenaline (Norepinephrine) ELISA Kit

INTENDED USE

Human noradrenaline ELISA kit is intended for measuring in vitro quantitative levels of noradrenaline or norepinephrine in human urine and plasma samples. This assay has a minimum detection limit of 1.7 ng/ml (U) and 0.04 pg/ml (P).

BACKGROUND

Noradrenaline (also called norepinephrine) is a member of the catecholamine family of hormones and neurotransmitters which has many vital functions in the brain and the body. Within the brain, norepinephrine is found to be formed in the nuclei or closely packed brain cell neurons which are yet too small to exert any meaningful effects on other areas of the brain. Outside the brain, norepinephrine is primary functions as a neurotransmitter by sympathetic ganglia which is located in the abdomen or near the spinal cord, it can also be directly released into the bloodstream via the adrenal glands.

Noradrenaline acts on a specific target cell by binding to and activating certain noradrenergic receptors which are located on the cell surface, regardless of where and how it is released. Norepinephrine has a structure is very similar to epinephrine, the only difference is the presence of a methyl group attached to its nitrogen in epinephrine whereas in norepinephrine this methyl group is replaced by a hydrogen atom. One of the major functions of norepinephrine is to mobilise the body and in particular the brain for action. Within the brain, it is well known to promoting vigilance, increases arousal and alertness, focuses attention, increases restlessness or anxiety, enhances the formation and retrieval of memory. Whereas, in the rest of the body it is able to increase blood pressure or heart rate, reduces blood flow to the gastrointestinal system, increases blood flow to skeletal muscles, triggers the release of glucose from energy stores, inhibits gastrointestinal motility and voiding of the bladder. The release of norepinephrine is at its lowest during sleep, rises during wakefulness and can reach highest levels during situations of danger or stress, in the so-called fight-or-flight response.

NORADRENALINE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Adhesive Foil.
  • Wash Buffer (50x Concentrated).
  • Enzyme Conjugate.
  • Substrate: TMB Chromogenic.
  • Stop Solution.
  • Noradrenaline Microtiter Strips: Antigen pre-coated microwell plate.
  • Antiserum – Rabbit anti-noradrenaline antibody.
  • Adjustment Buffer.
  • Standards (A-F): Concentrations 0, 5, 20, 75, 250, 1,000 ng/ml
  • Acylation Buffer.
  • Acylation Reagent.
  • Assay Buffer.
  • Coenzyme: S-adenosyl-L-methionine.
  • Enzyme: Catechol-O-methyltransferase.
  • Extraction Buffer.
  • Extraction Plate.
  • Hydrochloric Acid.
  • Control 1.
  • Control 2.

SENSITIVITY

The minimum sensitivity level of norepinephrine or noradrenaline using current norepinephrine ELISA kit was approximately 0.04 pg/ml (P) or 1.7 ng/ml (U). The dynamic range for this assay is 5.0 – 1,000 ng/ml.

ASSAY CHARACTERISTICS

– Noradrenaline (Norepinephrine): ELISA
– Expected Reference Values: < 90 µg/day (Urine) and < 600 pg/mL (Plasma)
– Analytical Sensitivity (Limit of Detection): 1.5 ng/mL (Urine) and 50 pg/mL (Plasma)
– Analytical Sensitivity: Derivatized Noradrenaline (100%), Normetanephrine 0.48%), Derivatized Dopamine (0.2%), Derivatized Adrenaline (0.14%), 3-Methoxy-4-hydroxyphenylglycol (0.01%), – Metanephrine, Tyrosine, 3-Methoxytyramine, Tyramine, 3-Methoxy-4-hydroxymandelic acid, Phenylalanine, Caffeinic acid, Homovanillic acid, L-Dopa (< 0.003%).
– Intra Assay Precision: 9.8 – 16.1%
– Inter Assay Precision: 8.5 – 15.0%
– Linearity: 85 – 123% (Urine) and 84 – 123% (Plasma)
– Recovery: 83 – 115% (Urine) and 85 – 108% (Plasma)

REFERENCES

  1. Relationships between central nervous system noradrenergic function and plasma and urinary concentrations of norepinephrine metabolites. Adv Biochem Psychopharmacol. (1984) 39: 45-55. Review. Maas J.W.
  2. Determination of catecholamines in plasma and urine. Best Pract Res Clin Endocrinol Metab. (2013) 27 (5): 713-23. Review. Grouzmann E. and Lamine F.
  3. Assessment of O-methylated catecholamine levels in plasma and urine for diagnosis of autonomic disorders. Auton Neurosci. (2004) 116 (1-2): 1-10. Review. Oeltmann T., et al.
  4. Recent advances in methods for the analysis of catecholamines and their metabolites. Anal Bioanal Chem. (2006) 386 (3): 506-14. Review. Tsunoda M.
  5. The role of catecholamines in clinical medicine. Acta Med Scand Suppl. (1979) 624:9-18. Review. Christensen N.J.

ADDITIONAL INFORMATION

  • Full Name: Noradrenaline (Norepinerphrine) ELISA Kit
  • Reactivity: Human
  • Sample Type: Urine, Plasma
  • Sensitivity: 17.0 ng/ml (U), 0.04 pg/ml (P)

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