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Neuron Specific Enolase (NSE) ELISA Kit

  • Created on the 11 May, 2017.

BACKGROUND

Neuron specific enolase (NSE) which can also be called gamma-enolase (ENO-2) or 2-phospho-D-glycerate hydrolase, is an isoenzyme belonging to the enolase family that consists up of α, β and γ subunits either has a homo or heterodimer. NSE is a 78 kD gamma-homodimer and the majority of this enolase-isoenzyme can be found in neuroendocrine and neuronal tissues. It is essentially a glycolytic enzyme that is able to catalyse the conversion of 2-phosphoglycerate into phosphoenolpyruvate. The biological half-life of NSE in body fluids is about 24 hours and due to the organ-specificity and concentrations of NSE in serum or cerebrospinal fluid (CSF), elevated levels have been identified in diseases which result in relative rapid neuronal destruction. The measurement of NSE concentration in serum of CSF has been useful in assisting the differential diagnosis of a variety of neurodegenerative and neuron-destructive disorders. The NSE ELISA assay is not designed for screening test for neuroendocrine tumors but can be used to measure levels in established diagnosis. NSE is useful tumor marker for lung cancer patients.

INTENDED USE

Human NSE ELISA kit is a method for analysing in vitro quantitative amounts of neuron specific enolase (gamma-enolase, ENO-2, NSE) in human serum samples. This assay has a minimum sensitivity limit of 0.19 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of human gamma-enolase (ENO-2, NSE) using this human neuron-specific enolase ELISA kit was 0.19 ng/ml. The dynamic assay range for this kit is 7.5 – 115.0 ng/ml.

REFERENCES

  1. Neuron-Specific Enolase as a Biomarker: Biochemical and Clinical Aspects. Adv Exp Med Biol. (2015) 867: 125-43. Review. Isgrò M.A., et al.
  2. A systematic literature review of cerebrospinal fluid biomarkers in delirium. Dement Geriatr Cogn Disord. (2011) 32 (2): 79-93. Review. Hall R.J., et al.
  3. Antibodies to neuron-specific enolase for the delineation of the entire diffuse neuroendocrine system in health and disease. Semin Diagn Pathol. (1984) 1 (1): 59-70. Review. Carlei F. and Polak J.M.
  4. Clinical biochemistry of neuron specific enolase. Clin Chim Acta. (1989) 183 (1): 13-31. Review. Kaiser E., et al.
  5. Serum neurone-specific enolase and other neuroendocrine markers in lung cancer. Eur J Cancer. (1994) 30A (5): 574-6. Review. Ledermann J.A.

ADDITIONAL INFORMATION

  • Full Name: Neuron Specific Enolase (NSE) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.19 ng/ml

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