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Neuron Specific Enolase (NSE) ELISA Kit

INTENDED USE

Human NSE ELISA kit is a method for analysing in vitro quantitative amounts of neuron specific enolase (gamma-enolase, ENO-2, NSE) in human serum samples. This assay has a minimum sensitivity limit of 0.19 ng/ml.

BACKGROUND

Neuron specific enolase (NSE) which can also be called gamma-enolase (ENO-2) or 2-phospho-D-glycerate hydrolase, is an isoenzyme belonging to the enolase family that consists up of α, β and γ subunits either has a homo or heterodimer. NSE is a 78 kD gamma-homodimer and the majority of this enolase-isoenzyme can be found in neuroendocrine and neuronal tissues. It is essentially a glycolytic enzyme that is able to catalyse the conversion of 2-phosphoglycerate into phosphoenolpyruvate. The biological half-life of neuron specific enolase in body fluids is about 24 hours and due to the organ-specificity and concentrations of NSE in serum or cerebrospinal fluid (CSF), elevated levels have been identified in diseases which result in relative rapid neuronal destruction.

The following NSE assay is not designed for screening test for neuroendocrine tumors but can be used to measure levels in established diagnosis. Neuron specific enolase is useful tumor marker for lung cancer patients. The measurement of NSE concentration in serum of CSF has been useful in assisting the differential diagnosis of a variety of neurodegenerative and neuron-destructive disorders.

NSE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C in the dark, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microplate: Monoclonal anti hNSE antibody adsorbed on the microwells.
  • Standards (0-4): Lyophilized, concentration 0, 4, 20, 50, 100 ng/ml.
  • Control Negative (Lyophilized).
  • Positive Control (Lyophilized).
  • Incubation Buffer.
  • Conjugate: Monoclonal anti hNSE antibody conjugated with horseradish peroxidase (HRP).
  • TMB-Substrate.
  • Stop Solution: Contains sulphuric acid, avoid any skin contact.
  • Wash Solution (50x Concentrate).

SENSITIVITY

The minimum detection sensitivity level of neuron specific enolase (ENO-2, gamma-enolase and NSE) using current human NSE ELISA kit was 0.19 ng/ml. The dynamic range for this assay is 7.5 – 115.0 ng/ml.

ASSAY CHARACTERISTICS

– Neuron Specific Enolase (NSE): ELISA
– Reference Serum Values (hNSE): Normal range (0 – 12 ng/mL) and Pathological value (> 12 ng/mL).
– Specificity: The antibody is directed specifically against the human neuron specific enolase.
– Sensitivity: 0.19 ng/mL
– Precision Intra-Assay: ≤ 4.4% (16x replicate measurements).
– Precision Inter-Assay: ≤ 11.2% (10x replicate measurements).
– Hook Effect: No Hook Effect up to 5000 ng/mL of hNSE.

REFERENCES

  1. Neuron Specific Enolase as a Biomarker: Biochemical and Clinical Aspects. Adv Exp Med Biol. (2015) 867: 125-43. Review. Isgrò M.A., et al.
  2. A systematic literature review of cerebrospinal fluid biomarkers in delirium. Dement Geriatr Cogn Disord. (2011) 32 (2): 79-93. Review. Hall R.J., et al.
  3. Antibodies to neuron specific enolase for the delineation of the entire diffuse neuroendocrine system in health and disease. Semin Diagn Pathol. (1984) 1 (1): 59-70. Review. Carlei F. and Polak J.M.
  4. Clinical biochemistry of neuron specific enolase. Clin Chim Acta. (1989) 183 (1): 13-31. Review. Kaiser E., et al.
  5. Serum neurone-specific enolase and other neuroendocrine markers in lung cancer. Eur J Cancer. (1994) 30A (5): 574-6. Review. Ledermann J.A.

ADDITIONAL INFORMATION

  • Full Name: Neuron Specific Enolase (NSE) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.19 ng/ml

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