GET A QUOTE

Nephrines Urine (Metanephrine + Normetanephrine) ELISA Kit

  • Created on the 4 March, 2017.

BACKGROUND

Metanephrine (M) and normetanephrine (NM) both contain a common structure of a methylated catechol ring (3-methoxy 4-hydroxy phenyl) in conjunction with an ethylamine group. Both of these are also O-methylated metabolites of the catecholamines epinephrine (E) and norepinephrine (NE) respectively. However, metanephrine is known to have a higher molecular weight of approx. 197 g/mol whereas NM is 183 g/mol. The only reason for this difference is because it contains a methylated amino group.

For clinical medicine metanephrine and normetanephrine have played essential roles in helping to identify individuals that could be possible suspects for possessing tumors of chromaffin (e.g. pheochromocytoma and paraganglioma). These metanephrines are also helpful as markers for extraneuronal metabolism of the parent catecholamines.

Both of the metabolites are routinely analysed in either plasma or urine samples, where it is only recently there has been an emergence of accurate and reliable procedure for determining plasma free metanephrines. Whereas the measurement of urine conjugated metanephrines have been used for quite a long time now and also is a very well established procedure.

INTENDED USE

Nephrines urine ELISA kit is intended for detecting in vitro quantitative concentrations of metanephrine (M) and normetanephrine (NM) in human urine samples. This assay has a minimum detection limit of 13.0 pg/ml (M) and 23.0 pg/ml (MN).

SENSITIVITY

The minimum sensitivity level of metanephrine (M) and normetanephrine (NM) using this human nephrines urine ELISA kit was approximately 13.0 pg/ml (M) or 23.0 pg/ml (MN). The dynamic assay range for this kit is 20.0 – 2,000 pg/ml (M) and 30.0 – 3,000 pg/ml (NM).

REFERENCES

  1. Deconjugated urinary metanephrine, normetanephrine and 3-methoxytyramine in laboratory diagnosis of pheochromocytoma and paraganglioma. Physiol Res. (2015) 64 Suppl 2: S313-22. Bílek R., et al.
  2. Diagnostic accuracy of free and total metanephrines in plasma and fractionated metanephrines in urine of patients with pheochromocytoma. Eur J Endocrinol. (2010) 162 (5): 951-60. Grouzmann E., et al.
  3. Urinary and plasma catecholamines and urinary catecholamine metabolites in pheochromocytoma: diagnostic value in 19 cases. Clin Chem. (1994) 40 (2): 250-6. Gerlo E.A. and Sevens C.
  4. Precisely wrong? Urinary fractionated metanephrines and peer-based laboratory proficiency testing. Clin Chem. (2005) 51 (2): 472-3. Singh R.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Nephrines Urine (Metanephrine + Normetanephrine) ELISA Kit
  • Reactivity: Human
  • Sample Type: Urine
  • Sensitivity: 13.0 pg/ml (M) and 23 pg/ml (NM)

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford