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N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) ELISA Kit

INTENDED USE

Human NT-proBNP ELISA kit can be used for measuring in vitro quantitative levels of N-terminal pro-brain natriuretic peptide, NT proBNP, NT-proBNP, BNPT) in human cell culture supernatant, plasma, cell lysates, serum and many other biological fluids. This assay has a minimum sensitivity limit of 2.0 pg/ml.

BACKGROUND

N-terminal prohormone of brain natriuretic peptide (also called NT-proBNP, BNPT and NT proBNP) is an inactive protein which is released as a result of cleaving the N-terminal 76 amino acid fragment from brain natriuretic peptide. Both the bioactive BNP and the inactive NT proBNP have been useful in the diagnosis and management of heart failure.

Their concentrations in blood are vital for the diagnosis and screening of acute congestive heart failure and can help to establish the prognosis in heart failure, since both of these marker are often elevated in patients with the worse outcome. The plasma levels are also increased in patients with associated coronary artery disease, myocardial ischemia, symptomatic or asymptomatic left ventricular dysfunction. Some of the main advantages NT-proBNP have over BNP is that it possesses superior stability, higher circulating levels and a relatively smaller amount of serum is needed to carry out a measurement.

NT-PROBNP ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microwell Plate: Anti-NT-proBNP polyclonal antibody is precoated onto 96-well plates.
  • Standards: Concentrations 62.5, 125, 250, 500, 1,000, 2,000, 4,000 pg/ml.
  • Sample/Standard Diluent Buffer.
  • HRP Conjugated Antibody (Dilution 1:100)(Do Not Freeze).
  • Antibody Diluent Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human N-terminal pro-brain natriuretic peptide (NT-proBNP, NT proBNP) using current NT-proBNP ELISA kit was 2.0 pg/ml. The dynamic range for this assay is 62.5 – 2,000.0 pg/ml.

ASSAY CHARACTERISTICS

– N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP)): ELISA
– Intra Assay Precision: < 10%
– Inter Assay Precision: < 10%

REFERENCES

  1. BNP and NT-proBNP as prognostic markers in persons with acute decompensated heart failure: a systematic review. Heart Fail Rev. (2014) 19 (4): 453-70. Review. Santaguida P.L., et al.
  2. The predictive value of plasma biomarkers in discharged heart failure patients: role of plasma NT-proBNP. Minerva Cardioangiol. (2016) 64 (2): 157-64. Review. Leto L., et al.
  3. Amino-Terminal Pro-B-Type Natriuretic Peptide for Diagnosis and Prognosis in Patients With Renal Dysfunction: A Systematic Review and Meta-Analysis. JACC Heart Fail. (2015) 3 (12): 977-89. Review. Schaub J.A., et al.
  4. The potential role of natriuretic peptides and other biomarkers in heart failure diagnosis, prognosis and management. Expert Rev Cardiovasc Ther. (2015) 13 (9): 1017-30. Review. Ibrahim N. and Januzzi J.L.
  5. N-terminal pro-B-type natriuretic peptide variability in stable dialysis patients. Clin J Am Soc Nephrol. (2015) 10 (4): 620-9. Fahim M.A. et al.

ADDITIONAL INFORMATION

  • Full Name: N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Lysates, Cell Culture Supernatant, Plasma, Serum, Other Biological Fluids
  • Sensitivity: 2.0 pg/ml

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