Rat N-ERC/Mesothelin ELISA Kit

Full Name: Rat N-ERC/Mesothelin ELISA Kit
Reactivity: Rat
Sample Type: Serum, Plasma, Ascites, Cell Culture Supernatant
Sensitivity: 10.0 pg/ml

BACKGROUND

ERC was initially identified to be expressed greatly in cancer affected renal tissue when compared to normal renal tissues in Eker rats. Mesothelin or MPF (megakaryocyte potentiating factor) are the equivalent human homologue of this protein. Mesothelin is up-regulated in renal cell carcinoma (RCC) and the protein has been associated with cancer metastasis. ERC is a protein that is located on cells’ surfaces. A key factor in mesothelin function is its ability to bind to the cell surface receptor, MERTK. This interaction can lead to cancer cell proliferation and metastasis.

It has been proposed that MPF might be a viable therapeutic target for stopping RCC from spreading through the bloodstream or tissue because this protein has been linked to both cancer growth and metastasis. This protein also inhibits angiogenesis, metastasis and invasion of “Diethyldithiocarbamate” (DDC) treated mice with lung adenocarcinoma. Another study found that MPF also suppresses RCC growth in vitro and in vivo by inducing apoptosis. Initiation of cell proliferation is regulated through cyclin D expression and activation of cyclin D dependent kinases that regulate cell cycle progression from G1/S phase to DNA synthesis.

ERC is found to be expressed as a GPI anchor-type membrane protein that is 71 kDa in size, which is digested by furin like proteinase in order to release a 31 kDa and 40 kDa fragment. It is expressed as a 71 kDa GPI anchor-type membrane protein which is often digested to release 31 kDa and 40 kDa fragments. The following procedure is ideal for measuring many types of diagnostic applications. This assay is intended to detect the 31 kDa fragment size which is referred to as N-ERC and mesothelin.

INTENDED USE

Rat N-ERC/Mesothelin ELISA kit is a procedure designed for quantitative detection of rat N-ERC or mesothelin (N-ERC/Mesothelin) using samples of EDTA plasma, ascites, serum and cell culture supernatant. This assay has a minimum sensitivity detection limit of 10.0 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Pre-Coated Plate: Anti-rat ERC (30F2) mouse IgG.
Labelled Antibody HRP Conjugated (30x Concentrated).
EIA Buffer.
Standard.
Solution For Labelled Antibody.
TMB Chromogen.
Wash Buffer (40x Conc.).
Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of N-ERC and mesothelin using current rat N-ERC/Mesothelin ELISA kit is 10.0 pg/ml. The dynamic range for this assay is 0.08 – 5.0 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 95.1 – 109.9%
– Added Recovery Assay: 87.0 – 98.4%
– Intra – Assay: 4.2 – 4.5%
– Inter – Assay: 4.2 – 5.5%
– Specificity: Rat N-ERC/Mesothelin (100.0%), Rat MCP-1 (≦0.1 %), Rat TNF-α (≦0.1 %), Rat Osteopontin (≦0.1 %).
– Sensitivity: 0.01 ng/mL

REFERENCES

Rat N-ERC/mesothelin as a marker for in vivo screening of drugs against pancreas cancer. PLoS One. (2014) 9 (10): e111481. Fukamachi K., et al.
Diagnostic tumor marker of asbestos-related mesothelioma. Environ Health Prev Med. (2008) 13 (2): 71-4. Hino O. and Maeda M.
Serum level of expressed in renal carcinoma (ERC)/ mesothelin in rats with mesothelial proliferative lesions induced by multi-wall carbon nanotube (MWCNT). J Toxicol Sci. (2010) 35 (2): 265-70. Sakamoto Y., et al.
Molecular tumor markers for asbestos-related mesothelioma: serum diagnostic markers. Pathol Int. (2006) 56 (11): 649-54. Review. Maeda M. and Hino O.

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