Myoglobin ELISA Kit (MB)

INTENDED USE

Human myoglobin ELISA kit is a protocol for determining in vitro quantitative amounts of myoglobin (MB) in human serum. This assay has a minimum sensitivity limit of 0.059 ng/ml.

BACKGROUND

Myoglobin (MB) is a small cytoplasmic heme-containing protein which consists of a single 153 amino acids polypeptide chain with an approx. molecular weight of 17kDa. It is a globular structure protein which is made up of eight alpha-helicies that are connected through the turns with an oxygen binding site. One of the major function is as an oxygen storage unit, provide oxygen to both the cardiac and skeletal muscles. It is able to bind oxygen with its heme group that is very similar to that of haemoglobin.

However, it only has one heme group whereas hemoglobin has four but the myoglobin does have a higher affinity for oxygen when compared to haemoglobin. This difference can be explained by the diferent roles each of these protein performs for example myoglobin’s function is primarily to store oxygen whereas haemoglobin is responsible for transporting oxygen. When the muscle tissues become damaged, there is evidence that a considerable quantity of it enters the kidneys. This can lead to acute renal failure because it can be highly toxic, also muscle injury has been linked to myoglobin being released and this has been found to cause myoalgia or even heart attacks.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microwell Plate: Antibody specific to murine monoclonal anti-myoglobin is pre-coated onto a 96-well plate.
• Reference Standard Set: Concentrations 0, 25, 100, 250, 500, 1000 ng/mL.
• Sample Diluent.
• Enzyme Conjugate Reagent.
• TMB Substrate.
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human myoglobin (MB) using this myoglobin ELISA kit was 5.0 ng/mL. The dynamic assay range for this kit is 0 – 1000.0 ng/mL.

ASSAY CHARACTERISTICS

– Clinical Performance: Correlation Coefficinent (0.9392), Slope (0.8871), Intercept (55.051), Mean (287.9 ng/mL), Abbott Kit Mean (262.5 ng/mL).
– Sensitivity: 5 ng/mL.
– Hook Effect: None up to concentrations 10,000 ng/mL.
– Intra-Assay Precision: 3.9 – 6.6%
– Inter-Assay Precision: 5.2 – 11.8%
– Recovery: 89.3 – 114.6%
– Linearity: 86.5 – 125.0%
– Specificity: Highly specific for myoglobin, no cross reaction with the following substances. Interfering Substances such as: Cardiac (TnI, TnT, TnC), Skeletal TnI, CK-MB, CK-MB2, CK-MM, CK-BB, Tropomysin, Myosin Light Chain Kinase (MLCK), Actin. Endogenous Substances such as: Billirubin, Cholesterol, Triglyceride, Total Protein. Therapeutic Substances such as: Aspirin, Coumadin, Digoxin, Flurosemide (Lasix), Sodium Heparin.

REFERENCES

1. Myoglobin_functions in the heart. Free Radic Biol Med. (2014) 73: 252-9. Review. Hendgen-Cotta U.B., et al.
2. Hemoglobin and myoglobin-associated oxidative stress: from molecular mechanisms to disease States. Curr Med Chem. (2005) 12 (23): 2741-51. Review. Reeder B.J. and Wilson M.T.
3. Keeping the heart in balance: the functional interactions of myoglobin with nitrogen oxides. J Exp Biol. (2010) 213 (Pt 16): 2726-33. Review. Flögel U., et al.
4. Redox reactions of hemoglobin and myoglobin: biological and toxicological implications. Antioxid Redox Signal. (2001) 3 (2): 313-27. Review. Alayash A.I., et al.

ADDITIONAL INFORMATION

• Full Name: Myoglobin ELISA Kit (MB)
• Reactivity: Human
• Sample Type: Serum
• Sensitivity: 5.0 ng/mL

OTHER RELATED ELISA KITS

• Troponin T ELISA Kit
• Creatine Kinase MB ELISA Kit
• Atrial Natriuretic Peptide ELISA Kit