- Created on the 16 May, 2017.
Human myoglobin ELISA kit is a protocol for determining in vitro quantitative amounts of myoglobin (MB) in human serum, cell culture supernatant, cell lysates, plasma and many other biological fluids. This assay has a minimum sensitivity limit of 1.03 ng/ml.
Myoglobin (MB) is a small cytoplasmic heme-containing protein which consists of a single 153 amino acids polypeptide chain with an approx. molecular weight of 17kDa. It is a globular structure protein which is made up of eight alpha-helicies that are connected through the turns with an oxygen binding site. One of the major function of myoglobin is as an oxygen storage unit, provide oxygen to both the cardiac and skeletal muscles. It is able to bind oxygen with its heme group that is very similar to that of haemoglobin.
However, it only has one heme group whereas hemoglobin has four but the myoglobin does have a higher affinity for oxygen when compared to haemoglobin. This difference can be explained by the diferent roles each of these protein performs for example myoglobin’s function is primarily to store oxygen whereas haemoglobin is responsible for transporting oxygen. When the muscle tissues become damaged, there is evidence that a considerable quantity of myoglobin enters the kidneys. This can lead to acute renal failure because it can be highly toxic, also muscle injury has been linked to myoglobin being released and this has been found to cause myoalgia or even heart attacks. Myoglobin ELISA kit is designed to be used for many different diagnostic applications.
The minimum detection sensitivity level of myoglobin (MB) using current human myoglobin ELISA kit was 1.03 ng/ml. The dynamic range for this assay is 2.0 – 600.0 ng/ml.
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- Full Name: Myoglobin (MB) ELISA Kit
- Reactivity: Human
- Sample Type: Cell Culture Supernatant, Plasma, Cell Lysates, Other Biological Fluids, Serum
- Sensitivity: 1.03 ng/ml