Myoglobin (MB) ELISA Kit

  • Created on the 16 May, 2017.


Human myoglobin ELISA kit is a protocol for determining in vitro quantitative amounts of myoglobin (MB) in human serum, cell culture supernatant, cell lysates, plasma and many other biological fluids. This assay has a minimum sensitivity limit of 1.03 ng/ml.


Myoglobin (MB) is a small cytoplasmic heme-containing protein which consists of a single 153 amino acids polypeptide chain with an approx. molecular weight of 17kDa. It is a globular structure protein which is made up of eight alpha-helicies that are connected through the turns with an oxygen binding site. One of the major function of myoglobin is as an oxygen storage unit, provide oxygen to both the cardiac and skeletal muscles. It is able to bind oxygen with its heme group that is very similar to that of haemoglobin.

However, it only has one heme group whereas hemoglobin has four but the myoglobin does have a higher affinity for oxygen when compared to haemoglobin. This difference can be explained by the diferent roles each of these protein performs for example myoglobin’s function is primarily to store oxygen whereas haemoglobin is responsible for transporting oxygen. When the muscle tissues become damaged, there is evidence that a considerable quantity of myoglobin enters the kidneys. This can lead to acute renal failure because it can be highly toxic, also muscle injury has been linked to myoglobin being released and this has been found to cause myoalgia or even heart attacks. Myoglobin ELISA kit is designed to be used for many different diagnostic applications.


The minimum detection sensitivity level of myoglobin (MB) using current human myoglobin ELISA kit was 1.03 ng/ml. The dynamic range for this assay is 2.0 – 600.0 ng/ml.


  1. Myoglobin functions in the heart. Free Radic Biol Med. (2014) 73: 252-9. Review. Hendgen-Cotta U.B., et al.
  2. Molecular insights into the functional role of myoglobin. Adv Exp Med Biol. (2007) 618: 181-93. Review. Garry D.J. and Mammen P.P.
  3. Hemoglobin and myoglobin associated oxidative stress: from molecular mechanisms to disease States. Curr Med Chem. (2005) 12 (23): 2741-51. Review. Reeder B.J. and Wilson M.T.
  4. Keeping the heart in balance: the functional interactions of myoglobin with nitrogen oxides. J Exp Biol. (2010) 213 (Pt 16): 2726-33. Review. Flögel U., et al.
  5. Redox reactions of hemoglobin and myoglobin: biological and toxicological implications. Antioxid Redox Signal. (2001) 3 (2): 313-27. Review. Alayash A.I., et al.


  • Full Name: Myoglobin (MB) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Plasma, Cell Lysates, Other Biological Fluids, Serum
  • Sensitivity: 1.03 ng/ml



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford