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Myeloperoxidase (MPO) ELISA Kit

  • Created on the 28 March, 2017.

BACKGROUND

Myeloperoxidase (MPO) is a cationic homodimer which is made up of two 15-kDa light chains and two variable-weight glycosylated heavy chains that are bound to a prosthetic heme group. It is essentially a lysosomal protein which is stored in azurophilic granules of neutrophil and then can be released into the extracellular space during the process of degranulation. MPO is abundantly expressed in neutrophils granulocytes and is secreted when they are activated, it is a mammalian phagocyte hemoprotein which can function in mediating host defence reactions. Intra-nuclear MPO can also help to protect DNA against damage as a result from oxygen radicals that are produced during myeloid cell function and maturation.

MPO is a member of the XPO subfamily of peroxidases where it has the ability to produce hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil’s respiratory burst. Both hypochlorous acid and tyrosyl radical are cytotoxic and they are used by the neutrophil to aid in killing bacteria and many other pathogens. However, there is also evidence that hypochlorous acid is able to result in oxidative damage within host tissue. Where it has been found that MPO oxidation of apoA-I can lead to a reduction in HDL-mediated inhibition of inflammation and apoptosis. Other functions of MPO include mediating protein nitrosylation   and the formation of dityrosine crosslinks and 3-chlorotyrosine.

INTENDED USE

Human myeloperoxidase ELISA kit is designed for determining in vitro quantitative amounts of myeloperoxidase (MPO) present in human plasma, serum, saliva, urine, tissue homogenate and cell culture supernatant. This assay has a minimum detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of MPO using this human  myeloperoxidase ELISA kit was 10.0 pg/ml. The dynamic assay range for this kit is 312.0 – 20,000 pg/ml.

REFERENCES

  1. Myeloperoxidase: a front-line defender against phagocytosed microorganisms. J Leukoc Biol. (2013) 93 (2): 185-98. Review. Klebanoff S.J., et al.
  2. Natural and disease associated anti-myeloperoxidase (MPO) autoantibodies. Autoimmun Rev. (2008) 7 (6): 421-5. Review. Guilpain P., et al.
  3. Human myeloperoxidase in innate and acquired immunity. Arch Biochem Biophys. (2010) 500 (1): 92-106. Review. Arnhold J. and Flemmig J.
  4. Coexistence of anti-glomerular basement membrane antibodies and myeloperoxidase-ANCAs in crescentic glomerulonephritis. Am J Kidney Dis. (2005) 46 (2): 253-62. Review. Rutgers A., et al.
  5. A retrospective study on the outcomes of MPO-ANCA-associated vasculitis in dialysis-dependent patients. Mod Rheumatol. (2016) 26 (1): 110-4. Hasegawa M., et al.
  6. Myeloperoxidase: a target for new drug development? Br J Pharmacol. (2007) 152 (6): 838-54. Review. Malle E., et al.

ADDITIONAL INFORMATION

  • Full Name: Myeloperoxidase (MPO) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant. Saliva, Plasma, Urine, Tissue Homogenate
  • Sensitivity: < 10.0 pg/ml

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