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Mustard ELISA Kit

  • Created on the 12 October, 2017.

BACKGROUND

Mustard is a condiment which can be prepared from mustard seeds and these can be classified into Brassicaceae (Cruciferae): Brassica juncea (brown), Brassica nigra (black), Sinapsis alba (yellow) varieties. It is often used in many different kinds of sauces and seasonings, this can result in masked allergen which can lead to allergic reactions. For a large number of mustard allergic people any hidden mustard allergens in food can cause them critical problems, there is evidence to indicate that even a very low amounts of mustard can lead to allergic reactions. This means that a mustard allergic individual must strictly avoid eating mustard or mustard containing food. Diagnostic procedures are often using prick-tests for mustard seeds and the detection of allergy antibodies is usually performed using blood samples. This ELISA assay offers a highly sensitive system to quantify levels of yellow mustard present in residues of sausage, cheese, soups, mixed herbs and dressings. Also, due to high cross-reactivity this assay is suitable for analysing black and brown mustard.

INTENDED USE

Mustard ELISA kit is designed for measuring quantitative amounts of mustard residues present in food (such as cheese, sausage, mixed herbs, dressings and soups). This assay has a minimum analytical sensitivity limit of 1.0 ppm.

SENSITIVITY

The minimum detection sensitivity level of mustard residues using this mustard ELISA kit was 1.0 ppm. The standard assay range for this kit is 2.0 – 60.0 ppm.

REFERENCES

  1. ELISA kit for mustard protein determination: interlaboratory study. J AOAC Int. (2011) 94 (2): 605-10. Cuhra P., et al.
  2. Competitive immunochromatographic assayfor the detection of thiodiglycol sulfoxide, a degradation product of sulfur mustard. Analyst. (2014) 139 (20): 5118-26. Sathe M., et al.
  3. Allergenicity assessment of transgenic mustard (Brassica juncea) expressing bacterial codA gene. Allergy. (2006) 61 (4): 491-7. Singh A.K., et al.
  4. Sandwich enzyme-linked immunosorbent assay (ELISA) for detection of mustard in foods. J Food Sci. (2008) 73 (4): T62-8. Lee P.W., et al.
  5. Validation and comparison of two commercial ELISA kits and three in-house developed real-time PCR assays for the detection of potentially allergenic mustard in food. Food Chem. (2015) 174: 75-81. Palle-Reisch M., et al.

ADDITIONAL INFORMATION

  • Full Name: Mustard ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.0 ppm

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