Mustard ELISA Kit

  • Created on the 12 October, 2017.


Mustard ELISA kit is designed for measuring quantitative amounts of mustard residues present in food (such as cheese, sausage, mixed herbs, dressings and soups). This assay has a minimum analytical sensitivity limit of 1.0 ppm.


Mustard is a condiment which can be prepared from mustard seeds and these can be classified into Brassicaceae (Cruciferae): Brassica juncea (brown), Brassica nigra (black), Sinapsis alba (yellow) varieties. It is often used in many different kinds of sauces and seasonings, this can result in masked allergen which can lead to allergic reactions. For a large number of mustard allergic people any hidden mustard allergens in food can cause them critical problems, there is evidence to indicate that even a very low amounts of mustard can lead to allergic reactions. This means that a mustard allergic individual must strictly avoid eating mustard or mustard containing food. Diagnostic procedures are often using prick-tests for mustard seeds and the detection of allergy antibodies is usually performed using blood samples. The following mustard ELISA kit offers a highly sensitive system to quantify levels of yellow mustard present in residues of sausage, cheese, soups, mixed herbs and dressings. Also, due to high cross-reactivity this assay is suitable for analysing black and brown mustard.


The minimum detection sensitivity level of mustard residues using current mustard ELISA kit was 1.0 ppm. The standard range for this assay is 2.0 – 60.0 ppm.


  1. ELISA kit for mustard protein determination: interlaboratory study. J AOAC Int. (2011) 94 (2): 605-10. Cuhra P., et al.
  2. Competitive immunochromatographic assayfor the detection of thiodiglycol sulfoxide, a degradation product of sulfur mustard. Analyst. (2014) 139 (20): 5118-26. Sathe M., et al.
  3. Allergenicity assessment of transgenic mustard (Brassica juncea) expressing bacterial codA gene. Allergy. (2006) 61 (4): 491-7. Singh A.K., et al.
  4. Sandwich enzyme-linked immunosorbent assay (ELISA) for detection of mustard in foods. J Food Sci. (2008) 73 (4): T62-8. Lee P.W., et al.
  5. Validation and comparison of two commercial ELISA kits and three in-house developed real-time PCR assays for the detection of potentially allergenic mustard in food. Food Chem. (2015) 174: 75-81. Palle-Reisch M., et al.


  • Full Name: Mustard ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.0 ppm



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford