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Mumps IgG ELISA Kit

  • Created on the 6 September, 2017.

BACKGROUND

Mumps is highly contagious viral disease which usually spreads quickly among people living in close proximity. It is a linear negative sense, single stranded enveloped RNA virus, which can be transmitted by respiratory droplets or even direct contact with an infected individual. Mumps virus has only been infect and spread amongst humans. It can be prevented by infection of two doses of the mumps vaccine, this is included in the immunisation program for many developed countries. It is often give in combination with measles, rubella and varicella vaccine. The identification of mumps in the laboratory is usually carried out by detecting the infectious agent itself or through the identification of virus-specific antibodies. Besides the classical methods such as fixation, neutralisation and hemagglutination tests, there are now some modern procedures like enzyme immunoassay, radio immunoassay and immunofluorescence which are routinely being used.

INTENDED USE

Human mumps-IgG ELISA kit is a procedure designed for detecting in-vitro quantitative amounts of human IgG antibodies against mumps virus using serum and plasma. This assay has a minimum analytical sensitivity limit of 1.18 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human mumps virus IgG using this human mumps-IgG ELISA kit was 1.18 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.

REFERENCES

  1. Mumps virus infection in vaccinated patients can be detected by an increase in specific IgG antibodies to high titres: a retrospective study. Epidemiol Infect. (2014) 142 (11): 2388-96. Borgmann S., et al.
  2. Seropositivity rates for measles, mumps, and rubella IgG and costs associated with testing and revaccination. Clin Vaccine Immunol. (2013) 20 (3): 443-5. Shirts B.H., et al.
  3. Detection of IgG-class antibodies to measles, mumps, rubella, and varicella-zoster virus using a multiplex bead immunoassay. Diagn Microbiol Infect Dis. (2010) 67 (4): 346-9. Dhiman N., et al.
  4. Avidity of IgG antibodies against mumps, parainfluenza 2 and Newcastle disease viruses after mumps infection. J Virol Methods. (1986) 14 (1): 1-7. Lehtonen O.P. and Meurman O.H.
  5. Detection of measles, mumps, and rubella viruses. Methods Mol Biol. (2011) 665: 183-93. Tipples G. and Hiebert J.

ADDITIONAL INFORMATION

  • Full Name: Mumps IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.18 U/ml

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