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Mouse Osteopontin ELISA Kit

  • Created on the 1 February, 2017.

BACKGROUND

Osteopontin (OPN) is a secreted glycoprotein which is also called a urinary stone protein, the protein sequence reveals a 34,982kDa (approx.317 amino acids protein) which contains a hydrophobic signal sequence (16 amino acids) and a 33,352Da protein that is destined to undergo extensive post-translational modifications prior to being secretion from cells. Osteopontin was originally isolated from bone and is a highly acidic calcium-binding glycosylated phosphoprotein which is secreted by many cell types, for example; activated T cells, kidney tubule cells, vascular smooth muscle cells, osteoblasts and macrophages.

Osteopontin has been known to be susceptible to proteolytic fragmentation and there is evidence to suggest that this process may have a physiologic importance. A recent study shown that thrombin treatment caused an enhancement of OPN cell adhesive activity, indicating that OPN cleavage by thrombin could result in exposing a cryptic adhesive sequence. Also, more recently, it has been demonstrated that the amino-terminal OPN fragment possesses a cryptic binding site that is similar to α9β1 integrin. The standard product used in this kit is recombinant mouse Osteopontin (OPN) that consists of 284 amino acid sequence (31.5KDa molecular weight). As a result of glycosylation, the molecular mass becomes 65KDa. The ELISA kit can be specifically used for assaying mouse osteopontin (OPN) and should not be used to detect thrombin-cleaved mouse OPN fragments.

INTENDED USE

Mouse osteopontin ELISA kit is a reliable quantitative method for measuring osteopontin (OPN) in mouse serum, plasma, cell culture media, tissue lysates and body fluids. This assay has a sensitivity detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of osteopontin (OPN) using this mouse osteopontin ELISA kit was approximately 10.0 pg/ml. The dynamic assay range for this kit is 156 – 10,000 pg/ml.

REFERENCES

  1. Osteopontin as a potential diagnostic biomarker for ovarian cancer. JAMA (2002) 287 (13): 1671-1679. Kim J-H., et al.
  2. Enhanced local production of osteopontin in rheumatoid joints. J Rheumatol. (2002) 29 (10): 2061-2067. Ohshima S., et al.
  3. Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption. Proc. Natl. Acad. Sci. USA. (1999) 96: 8156-8160. Yoshitake H., Rittling S. R., Denhardt D. T., and Noda M.
  4. Antibodies to different peptides in osteopontin reveal complexities in the various secreted forms. J. Cell. Biochem. (2000) 77: 487-498. Kon S., et al.

ADDITIONAL INFORMATION

  • Full Name: Mouse Osteopontin ELISA Kit
  • Reactivity: Mouse
  • Sample Type: Serum, Plasma, Cell Culture Media, Tissue Lysates, Body Fluids
  • Sensitivity: < 10.0 pg/ml

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