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Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit

  • Created on the 18 March, 2017.

BACKGROUND

Monocyte chemotactic protein 3 (MCP-3) is now renamed as chemokine (C-C motif) ligand 7 (CCL7), recently purified from human osteosarcoma cell line and is a novel chemokine. Due to the fact that CCL7 contains two adjacent N-terminal cysteine residues in its mature protein, it is classified as a CC chemokines subfamily member. MCP-3 has the ability to bind and activate a large number of diverse inflammatory cell types via the interaction with multiple leukocyte receptors as well as it’s own receptor. Evidence indicates that MCP-3 does indeed use multiple receptors (which includes MCP-1, MIP-1a, RANTES) and can also bind with MIP-1ß receptor as well as other unique binding sites. CCL7 can specifically attracts monocytes and regulates macrophage function. CCL7 has been shown to interact with MMP2.

cDNA cloning of MCP-3 and structural analysis has revealed that it composed of 76 amino acid polypeptide with a molar mass of 8.5 kDa. It is part of a small family of inflammatory proteins that can be characterized by four conserved cysteine residues and it is located on the human chromosome 17. MCP-3 and MCP-1 display 71% a.a. homology, whilst it is also revealed to have 30% a.a homology with RANTES and MIP-1 and these all can activate T-cells, basophils, monocytes and eosinophils. MCP-3 can also promote the exocytosis of eosinophil granule proteins and stimulates the release of histamine from human basophils.

INTENDED USE

MCP-3 ELISA kit can be used to determine in vitro quantitative levels of monocyte chemotactic protein 3 (MCP-3)(CCL7) in human serum, plasma,  cell culture supernatant and many other biological fluids. This assay has a minimum detection limit of < 30.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of MCP-3 (CCL7) using this human MCP-3 ELISA kit was 30.0 pg/ml. The dynamic assay range for this kit is 30.0 – 1,600 pg/ml.

REFERENCES

  1. Cytokines and chemoattractants in allergic inflammation. Mol Immunol. (2002) 38 (12-13): 881-5. Review. Romagnani S.
  2. Monocyte chemotactic protein-3. Eur Cytokine Netw. (2001) 12 (4): 554-60. Review. Menten P., et al.
  3. CC chemokines in allergic inflammation. Immunol Today. (1994) 15 (3): 127-33. Review. Baggiolini M. and Dahinden C.A.
  4. The role of CXCL12 and CCL7 chemokines in immune regulation, embryonic development, and tissue regeneration. Cytokine. (2014) 69 (2): 277-83. Review. Cheng J.W., et al.

ADDITIONAL INFORMATION

  • Full Name: Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum, Cell Culture Supernatant, Biological Fluids
  • Sensitivity: < 30.0 pg/ml

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