Monocyte Chemotactic Protein 1 (MCP-1) ELISA Kit
- Created on the 18 March, 2017.
INTENDED USE
MCP-1 ELISA kit is a protocol intended for analysing in vitro quantitative amounts of monocyte chemotactic protein 1 (MCP1)(MCAF, LDCF, GDF) in human serum, plasma, urine and cell culture supernatant. This assay has a minimum detection limit of < 1.0 pg/ml.
BACKGROUND
Monocyte chemotactic protein 1 (MCP1) which is also known as monocyte chemotactic and activating factor (MCAF), glioma-derived chemotactic factor (GDF) and lymphocyte–derived chemotactic factor (LDCF), is a newly identified chemotactic cytokine for monocytes. MCP-1 is composed of a 76-amino acid polypeptide with an approx. molecular mass of 8,700 Da and it belongs to a family low molecular weight structurally-related proteins that are characterised by four conserved cysteine residues (known as C-C or intercrine b family).
MCAF can be expressed by a number of different types of cultured cells such as lymphocytes, endothelial cells, monocytes, smooth muscle cells, fibroblasts and many transformed cell lines upon stimulation with cytokines or LPS. It is found to plays a crucial and unique function in the initiation of atherosclerosis and there is evidence that it may provide a new therapeutic target in this disorder. In addition to MCP-1 being chemotactic for monocytes, it can also activate human monocytes in order to become cytostatic for many human tumour cell lines, generate superoxide and release lysosomal enzymes. In order to further clarify and elucidate the participation and relation of MCAF with various disorders, an in vivo quantitative analysis of this protein is necessary. The following MCP-1 ELISA kit can be used for many diagnostic applications.
SENSITIVITY
The minimum detection sensitivity level of monocyte chemotactic protein 1 (MCP1, MCAF) using current human MCP-1 ELISA kit was 1.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.
REFERENCES
- Impact of genetic variations in C-C chemokine receptors and ligands on infectious diseases. Hum Immunol. (2016) 77 (10): 961-71. Review. Qidwai T. and Khan M.Y.
- Interleukin-8 and MCAF: novel leukocyte recruitment and activating cytokines. Chem Immunol. (1992) 51: 236-65. Review. Matsushima K., et al.
- Monocyte Chemoattractant Protein 1 (MCP-1) in obesity and diabetes. Cytokine. (2012) 60 (1): 1-12. Review. Panee J.
- Renaming cytokines: MCP-1, major chemokine in pancreatitis. Gut. (2005) 54 (12): 1679-81. Review. Marra F.
- Role of MCP-1 in cardiovascular disease: molecular mechanisms and clinical implications. Clin Sci (Lond). (2009) 117 (3): 95-109. Review. Niu J. and Kolattukudy P.E.
ADDITIONAL INFORMATION
- Full Name: Monocyte Chemotactic Protein 1 (MCP-1) ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Serum, Cell Culture Supernatant, Urine
- Sensitivity: < 1.0 pg/ml
