Mollusc (Tropomyosin) ELISA Kit


Mollusc (tropomyosin) ELISA kit can be used for detecting quantitative levels of tropomyosin and mollusc in food (such as fish, meat, bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.


Molluscs represent a large group of seafood’s which can include mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. It is also a food allergen since a large number of adverse reactions have been reported following the ingestion of molluscs, the allergic reactions found are similar to those found with other foods. Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts.

The following mollusc/tropomyosin ELISA kit represents a highly sensitive procedure that is able to quantify mollusc residues in fish products, meat products, bakery products, dressings and soups. A very small quantity of allergen is enough cause allergic reactions and in severe cases can result in anaphylactic shock. There is also the possibility of cross-reactions between molluscs in particular within the same class (for example cephalopods, bivalves and gastropods). Allergies to molluscs tend to be diagnosed in a similar fashion to other food allergies.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter plate consisting of 12 strips with 8 breakable wells: Coated with anti tropomyosin antibodies.
  • Tropomyosin Standards 1-5: Concentration 0-400 ppb.
  • Conjugate (anti tropomyosin peroxidase).
  • Substrate Solution (TMB).
  • Stop Solution (0.5 M H2SO4).
  • Extraction and sample dilution buffer (Tris)(10x).
  • Washing Solution (PBS + Tween 20), 10x concentrate.
  • Instruction Manual.


The minimum detection sensitivity level of mollusc and tropomyosin using current mollusc/tropomyosin ELISA kit was 1.7 ppb. The standard range for this assay is 10.0 – 400.0 ppb.


  1. Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure. Food Chem Toxicol. (2015) 76: 86-93. Jin Y., et al.
  2. Effect of pH shifts on IgE-binding capacity and conformational structure of tropomyosin from short-neck clam (Ruditapes philippinarum). Food Chem. (2015) 188: 248-55. Lin H., et al.
  3. Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon. (2016) 111: 121-9. Johnson S., et al.
  4. Cross-reactivity between storage and dust mites and between mites and shrimp. Exp Appl Acarol. (2009) 47 (2): 159-72. Arlian L.G., et al.
  5. A novel monoclonal antibody-based immunoenzymatic assay for epidemiological surveillance of the vector snails of Fasciola hepatica (Trematoda: Digenea). Int J Parasitol. (2015) 45 (2-3): 113-9. Alba A., et al.


  • Full Name: Mollusc (Tropomyosin) ELISA Kit
  • Sample Type: Food (Soups, Fish, Bakery, Dressings, Meat)
  • Sensitivity: 1.7 ppb



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