Mollusc (tropomyosin) ELISA kit can be used for detecting quantitative levels of tropomyosin and mollusc in food (such as fish, meat, bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.
Molluscs represent a large group of seafood’s which can include mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. It is also a food allergen since a large number of adverse reactions have been reported following the ingestion of molluscs, the allergic reactions found are similar to those found with other foods. Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts.
The following mollusc/tropomyosin ELISA kit represents a highly sensitive procedure that is able to quantify mollusc residues in fish products, meat products, bakery products, dressings and soups. A very small quantity of allergen is enough cause allergic reactions and in severe cases can result in anaphylactic shock. There is also the possibility of cross-reactions between molluscs in particular within the same class (for example cephalopods, bivalves and gastropods). Allergies to molluscs tend to be diagnosed in a similar fashion to other food allergies.
MOLLUSC (TROPOMYOSIN) ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter plate consisting of 12 strips with 8 breakable wells: Coated with anti tropomyosin antibodies.
- Tropomyosin Standards 1-5: Concentration 0-400 ppb.
- Conjugate (anti tropomyosin peroxidase).
- Substrate Solution (TMB).
- Stop Solution (0.5 M H2SO4).
- Extraction and sample dilution buffer (Tris)(10x).
- Washing Solution (PBS + Tween 20), 10x concentrate.
- Instruction Manual.
The minimum detection sensitivity level of mollusc and tropomyosin using current mollusc/tropomyosin ELISA kit was 1.7 ppb. The standard range for this assay is 10.0 – 400.0 ppb.
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- Effect of pH shifts on IgE-binding capacity and conformational structure of tropomyosin from short-neck clam (Ruditapes philippinarum). Food Chem. (2015) 188: 248-55. Lin H., et al.
- Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon. (2016) 111: 121-9. Johnson S., et al.
- Cross-reactivity between storage and dust mites and between mites and shrimp. Exp Appl Acarol. (2009) 47 (2): 159-72. Arlian L.G., et al.
- A novel monoclonal antibody-based immunoenzymatic assay for epidemiological surveillance of the vector snails of Fasciola hepatica (Trematoda: Digenea). Int J Parasitol. (2015) 45 (2-3): 113-9. Alba A., et al.
- Full Name: Mollusc (Tropomyosin) ELISA Kit
- Sample Type: Food (Soups, Fish, Bakery, Dressings, Meat)
- Sensitivity: 1.7 ppb