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Mollusc (Tropomyosin) ELISA Kit

  • Created on the 12 October, 2017.

BACKGROUND

Molluscs represent a large group of seafood’s which can include mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. It is also a food allergen since a large number of adverse reactions have been reported following the ingestion of molluscs, the allergic reactions found are similar to those found with other foods. Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts. A very small quantity of allergen is enough cause allergic reactions and in severe cases can result in anaphylactic shock. There is also the possibility of cross-reactions between molluscs in particular within the same class (for example cephalopods, bivalves and gastropods). Allergies to molluscs tend to be diagnosed in a similar fashion to other food allergies. The ELISA assay represents a highly sensitive procedure that is able to quantify mollusc residues in fish products, meat products, bakery products, dressings and soups.

INTENDED USE

Mollusc ELISA kit can be used for detecting quantitative levels of mollusc tropomyosin in food (such as fish, meat and bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.

SENSITIVITY

The minimum detection sensitivity level of mollusc tropomyosin using this mollusc ELISA kit was 1.7 ppb. The standard assay range for this kit is 10.0 – 400.0 ppb.

REFERENCES

  1. Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure. Food Chem Toxicol. (2015) 76: 86-93. Jin Y., et al.
  2. Effect of pH shifts on IgE-binding capacity and conformational structure of tropomyosin from short-neck clam (Ruditapes philippinarum). Food Chem. (2015) 188: 248-55. Lin H., et al.
  3. Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon. (2016) 111: 121-9. Johnson S., et al.
  4. Cross-reactivity between storage and dust mites and between mites and shrimp. Exp Appl Acarol. (2009) 47 (2): 159-72. Arlian L.G., et al.
  5. A novel monoclonal antibody-based immunoenzymatic assay for epidemiological surveillance of the vector snails of Fasciola hepatica (Trematoda: Digenea). Int J Parasitol. (2015) 45 (2-3): 113-9. Alba A., et al.

ADDITIONAL INFORMATION

  • Full Name: Mollusc (Tropomyosin) ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.7 ppb

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