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Metanephrine Urine ELISA Kit

INTENDED USE

Human metanephrine urine ELISA kit is designed for measuring in vitro quantitative levels of metanephrine (metaadrenaline) in human urine samples. This assay has a minimum detection level of 13.0 ng/ml.

BACKGROUND

Metanephrine (metaadrenaline) are classified as catecholamines hormone molecules and they can be produced as a metabolite of adrenaline (epinephrine). These hormones are vital in the regulation of blood flow and pressure throughout the body during periods of physical and emotional stress. These metanephrine hormones are useful in detecting tumors such as pheochromocytomas and paragangliomas, since these tumors are found to be producing these hormones in excess. Assays that are currently being used to measure the presence of catecholamine-secreting tumors (pheochromocytomas or paragangliomas) include urine catecholamines, plasma catecholamines, urine metanephrines and plasma free metanephrines. All of these procedures have varying specificity and sensitivity.

Urine metanephrine assays are used to measure the total amount of metanephrine that is released into the urine in a 24 hour period. Due to the fact that the concentrations of this hormone may fluctuate significantly throughout the day, the urine test may be able to detect excess production that could have been missed using the blood test. The current guidelines recommends using either/or both urinary fractionated metanephrine and plasma free metanephrine for initial biochemical testing.

METANEPHRINE URINE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Metanephrine Microtiter Strips.
  • Standards (A-F): Concentrations 0, 20, 60, 200, 600, 2,000 ng/ml (= µg/l).
  • Controls 1 + Control 2.
  • Metanephrine Antiserum.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Wash Buffer (50x Concentrate).
  • Stop Solution.
  • Reaction Tubes.
  • Acylation Diluent.
  • Acylation Concentrate.
  • Acylation Buffer.
  • Hydrochloric Acid.

SENSITIVITY

The minimum sensitivity limit of human metanephrine (metaadrenaline) using current metanephrine urine ELISA kit was approximately 13.0 ng/ml. The dynamic range for this assay is 20.0 – 2,-000 pg/ml.

ASSAY CHARACTERISTICS

– Metanephrine (Urine): ELISA
– Analytical Sensitivity: LOD (8.6 ng/ml), LOB (6.0 ng/ml) and LOQ (15.1 ng/ml).
– Analytical Specificity (Cross Reactivity): Derivatized Metanephrine (100%), Adrenaline (3.3%), Derivatized Normetanephrine (0.15%), Noradrenaline, 3-Methoxytyramine, L-Tyrosin, Vanillic mandelic acid, Dopamine, Tyramine, L-Dopa (< 0.01%).
– Intra Assay Precision: 9 – 12%
– Inter Assay Precision: 16 – 17%
– Linearity: 46.2 – 204 ng/ml
– Recovery: 20.2 – 1484 ng/ml

REFERENCES

  1. Consideration of the degree of increase in urine metadrenalines provides superior specificity in the diagnosis of phaeochromocytoma than additional urine catecholamine measurement. Ann Clin Biochem. (2013) 50 (Pt 1): 73-5. Scargill J.J., et al.
  2. Streamlining the imaging of clinically suspected pheochromocytoma: using urine metanephrines to decrease imaging costs. Can Assoc Radiol J. (2014) 65 (4): 372-8. Stamm M. and Abele J.T.
  3. Elevated urine metanephrines in catatonia: a forgotten measure? Psychosomatics. (2014) 55 (5): 521-3. Grubisha M., et al.
  4. Changes in urinary total metanephrine excretion in recurrent and malignant pheochromocytomas and secreting paragangliomas. Ann N Y Acad Sci. (2006) 1073: 383-91. Amar L., et al.
  5. Urinary free (unconjugated) metadrenalines in different hereditary forms of catecholamine-secreting phaeochromocytoma/paraganglioma. Ann Clin Biochem. (2012) 49 (Pt 5): 486-90. Davidson D.F., et al.

ADDITIONAL INFORMATION

  • Full Name: Metanephrine Urine ELISA Kit
  • Reactivity: Human
  • Sample Type: Urine
  • Sensitivity: 13.0 ng/ml

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