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Matrix Metalloproteinase-9 (MMP-9) ELISA Kit

  • Created on the 6 May, 2017.

BACKGROUND

Matrix metalloproteinase-9 (MMP-9) which can also be referred to as gelatinase B, type V collagenase or 92 kD type IV collagenase, are members of the group of secreted zinc metalloproteases. It is a matrixin which is involved in degrading extracellular matrix, allow angiogenesis and tumor invasion. Human MMP9 pro-enzyme consists of five domains: amino-terminal pro-peptide, carboxyl-terminal hemopexin-like domain (conserved) and the zinc-binding catalytic domain.

MMP9 is predominantly known to be expressed in macrophages, neutrophils and mast cells and together with elastase is a regulatory factor in the migration of neutrophil across the basement membrane. Gelatinase B, has been identified to have proteolytic activity against connective tissue proteins and it has be indicated to be vital in the process of connective tissue remodelling which is linked with plaque rupture and atherogenesis. Elevated levels of MMP-9 are discovered in cases of focal brain ischemia and rheumatoid arthritis.

INTENDED USE

Human MMP-9 ELISA kit can be used for measuring in vitro quantitative concentrations of matrix metalloproteinase-9 (gelatinase B, MMP9, 92 kD type IV collagenase or type V collagenase) in human cell lysates, serum, plasma, cell culture supernatant and many other biological fluids. This assay has a minimum sensitivity limit of 15.12 ng/mL.

SENSITIVITY

The minimum detection sensitivity level of human MMP-9 (type V collagenase, gelatinase B, 92 kD type IV collagenase) using this human matrix metalloproteinase-9 ELISA kit was 15.12 ng/mL. The dynamic assay range for this kit is 30.0 – 9,000 ng/mL.

REFERENCES

  1. On the structure and functions of gelatinase B/matrix metalloproteinase-9 in neuroinflammation. Prog Brain Res. (2014) 214: 193-206. Review. Vandooren J., et al.
  2. Epigenetic regulation of mmp-9 gene expression. Cell Mol Life Sci. (2013) 70 (17): 3109-24. Review. Labrie M. and St-Pierre Y.
  3. New strategies for targeting matrix metalloproteinases. Matrix Biol. (2015) 44-46: 239-46. Review. Fields G.B.
  4. MMP9: a novel function in synaptic plasticity. Int J Biochem Cell Biol. (2012) 44 (5): 709-13. Review. Dziembowska M. and Wlodarczyk J.
  5. A systematic review of matrix metalloproteinase 9 as a biomarker of survival in patients with osteosarcoma. Tumour Biol. (2014) 35 (6): 5487-91. Review. Li H., et al.
  6. Mmp-9 inhibition: a therapeutic strategy in ischemic stroke. Mol Neurobiol. (2014) 49 (1): 563-73. Review. Chaturvedi M. and Kaczmarek L.

ADDITIONAL INFORMATION

  • Full Name: Matrix Metalloproteinase-9 (MMP-9) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Other Biological Fluids, Plasma, Cell Culture Supernatant, Cell Lysates
  • Sensitivity: 15.12 ng/mL

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