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Matrix Metalloproteinase-12 (MMP-12) ELISA Kit

  • Created on the 6 May, 2017.

BACKGROUND

Matrix metalloproteinase-12 (also known as macrophage elastase, macrophage metalloelastase or MMP12) is encoded by a pre-proprotein which needs to be proteolytically processed in order to generate the mature protease. The active enzyme is composed of two domains, one which is the catalytic domain (enzymatic activity) and the other the hemopexin-like domain (substrate recognition).

MMP-12 is initially a proenzyme (54 kDa) that is processed into a 45 kDa and then later into a 22 kDa which represents the active form. MMP12 is predominately formed by macrophages, it has also been linked to atherosclerosis, inflammatory skin diseases, aneurysms and a number of other cancers. There is evidence to suggest that it may be critical mediator of acute and chronic lung injury. MMP-12 has been found to play important roles during aneurysm formation (mouse models only) and this has led to the belief that it could have a role during emphysema development (humans).

INTENDED USE

Human MMP-12 ELISA kit is intended for analysing in vitro quantitative levels of matrix metalloproteinase-12 (MMP12, macrophage elastase) in human plasma, cell culture supernatant, other biological fluids, cell lysates and serum. This assay has a minimum sensitivity limit of 0.09 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of human MMP-12 (macrophage elastase) using this human matrix metalloproteinase-12 ELISA kit was 0.09 ng/ml. The dynamic assay range for this kit is 0.2 – 60 ng/ml.

REFERENCES

  1. Complex role of matrix metalloproteinases in angiogenesis. Cell Res. (1998) 8 (3): 171-7. Review. Sang Q.X.
  2. Macrophage elastase (MMP-12): a pro-inflammatory mediator? Mem Inst Oswaldo Cruz. (2005) 100 Suppl 1: 167-72. Review. Nénan S., et al.
  3. Regulation of matrix metalloproteinases: an overview. Mol Cell Biochem. (2003) 253 (1-2): 269-85. Review. Chakraborti S., et al.
  4. New insights into the substrate specificity of macrophage elastase MMP-12. Biol Chem. (2016) 397 (5): 469-84. Lamort A.S., et al.

ADDITIONAL INFORMATION

  • Full Name: Matrix Metalloproteinase-12 (MMP-12) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Other Biological Fluids, Plasma, Cell Lysates, Serum
  • Sensitivity: 0.09 ng/ml

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