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MMP-11 ELISA Kit (Matrix Metalloproteinase-11)

INTENDED USE

Human MMP-11 ELISA kit can be used for detecting in vitro quantitative amounts of matrix metalloproteinase-11 (MMP-11, MMP11, stromelysin-3) in human cell culture supernatant, serum, cell lysates, plasma and other biological fluids. This assay has a minimum sensitivity limit of 0.094 ng/ml.

BACKGROUND

Matrix metalloproteinase-11 (MMP-11, stromelysin-3, MMP11, SL-3) is secreted by mesenchymal origin cells. The human form consists of 31 amino acids signal sequence, hemopexin-like domains, a pro-peptide that is cleaved at a furin consensus sequence and a 390 a.a. mature sequence. The mature human protein is 45 kDa and it displays 89% amino acids sequence identity with mouse.

Diseases that are associated with MMP-11 are colorectal cancer and basal cell carcinoma. It has been found to be upregulated in stromal cells that are located near invasive carcinomas, it is able to cleave IGFBP which can allow the free IGF-1 to promote tumor cell growth.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with MMP-11.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of matrix metalloproteinase-11 (MMP11, MMP-11, stromelysin-3) using current MMP-11  ELISA kit was 0.094 ng/ml. The dynamic range for this assay is 0.156 – 10 ng/ml.

REFERENCES

  1. Matrix metalloproteinase 11 (MMP-11; stromelysin-3) and synthetic inhibitors. Med Res Rev. (2007) 27 (4): 528-52. Review. Matziari M., et al.
  2. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. Int J Mol Sci. (2016) 17 (6). pii: E868. Review. Pittayapruek P., et al.
  3. Insights into the distinct roles of MMP-11 in tumor biology and future therapeutics (Review). Int J Oncol. (2016) 48 (5): 1783-93. Review. Zhang X., et al.
  4. Cancer cells, adipocytes and matrix metalloproteinase-11: a vicious tumor progression cycle. Biol Chem. (2008) 389 (8): 1037-41. Review. Motrescu E.R. and Rio M.C.

ADDITIONAL INFORMATION

  • Full Name: MMP-11 ELISA Kit (Matrix Metalloproteinase-11)
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Cell Lysates, Other Biological Fluids, Plasma, Serum
  • Sensitivity: 0.094 ng/ml

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