GET A QUOTE

Matrix Metalloproteinase-1 (MMP-1) ELISA Kit

  • Created on the 5 May, 2017.

BACKGROUND

Matrix metalloproteinase-1 (MMP-1) is also referred to as fibroblast collagenase or interstitial collagenase and is part of the MMP genes that can be found located on chromosome 11q22.3. MMPs are made up of around 20 proteolytic enzymes that are essential in tissue remodelling. MMP-1 is found to play a critical role in the degradation of collagen which is present in inflammatory diseases. It possesses an archetypal structure that is made up of a pre-domain, a catalytic domain, a hemopexin-like domain, a pro-domain and a linker region. The catalytic domain consists of 5 highly twisted beta-strands, 3 alpha-helix and an overall total of eight loops which enclose 5 metal ions, one of which with found to possess the catalytic role.

MMPs are important for breaking down of extracellular matrix in normal physiological processes (for example in tissue remodelling, reproduction and embryonic development) and also in many disease processes (for example metastasis and arthritis). MMP-1 are a specific function in the breakdown of interstitial collagens (I, II and III). It also has the ability to interact with CD49b and mechanical forces have been identified which can lead to increasing the expression of MMP1 in human periodontal ligament cells.

INTENDED USE

Human MMP-1 ELISA kit can be used for determining in vitro quantitative levels of matrix metalloproteinase-1 (interstitial or fibroblast collagenase) in human cell culture supernatant, serum, cell lysates, other biological fluids and plasma. This assay has a minimum sensitivity limit of 0.065 ng/mL.

SENSITIVITY

The minimum detection sensitivity level of human MMP-1 (fibroblast or interstitial collagenase) using this human matrix metalloproteinase-1 ELISA kit was 0.065 ng/mL. The dynamic assay range for this kit is 0.156 – 10 ng/mL.

REFERENCES

  1. Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. (2015) 53 (5): 585-600. Review. Craig V.J., et al.
  2. MMP-1 polymorphism and its relationship to pathological processes. J Biosci. (2009) 34 (2): 313-20. Review. Arakaki P.A., et al.
  3. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. Int J Mol Sci. (2016) 17 (6). pii: E868. Review. Pittayapruek P., et al.
  4. Membrane-type matrix metalloproteinases (MT-MMPs) in tumor metastasis. J Biochem. (1996) 119 (2): 209-15. Review. Sato H. and Seiki M.
  5. MMP-1: the elder of the family. Int J Biochem Cell Biol. (2005) 37 (2): 283-8. Review. Pardo A. and Selman M.

ADDITIONAL INFORMATION

  • Full Name: Matrix Metalloproteinase-1 (MMP-1) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Plasma, Cell Lysates, Other Biological Fluids, Serum
  • Sensitivity: 0.065 ng/mL

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford