GET A QUOTE

Luteinizing Hormone (LH) ELISA Kit

  • Created on the 17 February, 2017.

BACKGROUND

Luteinizing hormone (LH) is a glycoprotein which is produced in both females and males in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH) from the anterior pituitary gland. It has a molecular weight of 30,000 daltons and consists of alpha and beta chains that are associated non-covalently. The α-subunit is identical to the other pituitary hormones namely chorionic gonadotropin (hCG), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), whilst the β-subunit is unique and confers the biological activity to the molecule. The α -subunit is made up of 89 amino acid residues and the β-subunit consists of 129 amino acids, with the carbohydrate content being between 15%-30%.

The importance of determining luteinizing hormone (LH) is to ascertain the homeostasis of fertility regulation through the hypothalamic-pituitary-gonadal axis. Also, it can help the advent of in vitro fertilization (IVF) procedure in order to overcome infertility that is associated to various problems. Over the many year the vast improvement in LH ELISA methodology from bioassays that are technically very demanding to the many simple and rapid immunoassays have helped this cause considerably. In the differential diagnosis of pituitary, gonadal dysfunction or hypothalamic, a number of assays that measure the concentration of LH have been routinely used in conjunction with FSH due to the fact that their roles are inter-related. Other uses include pinpointing ovulation, determining menopause and monitoring endocrine therapy.

INTENDED USE

Luteinizing hormone ELISA kit is a reliable quantitative procedure for measuring luteinizing hormone (LH) in human serum samples. This assay has a minimum sensitivity detection limit of 1.2 mIU/ml.

SENSITIVITY

The minimum sensitivity detection limit of luteinizing hormone using this human luteinizing hormone ELISA kit was approximately 1.2 mIU/ml. The dynamic assay range for this kit is 10.0 – 200.0 mIU/ml.

REFERENCES

  1. Understanding the role of LH: myths and facts. Reprod Biomed Online. (2007) 15 (4): 468-77. Fischer R.
  2. Benefits of luteinizing hormone activity in ovarian stimulation for IVF. Reprod Biomed Online. (2009) 18 Suppl 2: 31-6. Review. Venetis C.A., et al.
  3. FSH and LH together in ovarian stimulation. Eur J Obstet Gynecol Reprod Biol. (2004) 115 Suppl 1: S34-9. Review. Levi-Setti P.E., et al.
  4. Pituitary effects of steroid hormones on secretion of follicle-stimulating hormone and luteinizing hormone. Domest Anim Endocrinol. (2002) 23 (1-2): 33-42. Review. Nett T.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Luteinizing Hormone (LH) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 1.2 mIU/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford